Examples of 'acylase' in a sentence
Meaning of "acylase"
The adjective 'acylase' is used in biology to describe enzymes that catalyze the hydrolysis of acyl compounds
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- Any enzyme that catalyses the removal of acyl groups, especially from acylated amino acids.
How to use "acylase" in a sentence
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acylase
The acylase elutes in equilibration buffer.
Then hydrogen atoms of the acylase were allowed to move.
A preferred method is enzymatic deacylation using a suitable dicarboxylate acylase.
Suitable gapped duplex molecules of acylase genes were constructed.
The enzyme may for example be a mutant penicillin acylase.
It showed that correct maturation of the acylase is very sensitive to mutagenesis.
The purified acylase is immobilized onto particles consisting of a mixture of gelatine and chitosan.
In the case of penicillin acylase.
The immobilised acylase and the liquid were separated with a glass sintered filter.
Construction of synthetic genes expressing amino acid acylase activity.
Wild type PenG acylase shows the highest activity for PenG as was expected.
Preparation of acylase.
The novel acylase elaborated is found in both the grown mycelium and the filtrate.
Such enzymes include the enzymes collectively referred to as penicillin amidase or penicillin acylase.
A nucleic acid sequence encoding a mutant acylase as defined in any one of the preceding claims.
See also
This is accomplished by adding into the culture medium a NAM specific acylase.
Penicillin acylase activity was assayed using NIPAB as a substrate.
After removing the cell debris the supernatants containing the acylase activity are collected.
Purification of the acylase from a recombinant Bacillus subtilis.
Specific enzyme activity The activity of the cephalosporin C acylase was determined by HPLC assay.
The new mutant CC acylase of this invention can be characterized by the following.
Immobilized Alcaligenes faecalis penicillin G acylase is removed by filtration.
The acylase is eluted in two peaks corresponding to the a and B subunit.
The following examples illustrate the acylase produced by Streptomyces sp.
Preferably, this hydrolysis is carried out with the enzyme penicillin acylase.
The immobilized penicillin V acylase was recovered by centrifugation.
Accordingly, the invention also concerns a microorganism expressing an acylase of the invention.
The results of the penicillin G acylase production are surprisingly good.
Subsequently, the aqueous phase or solution is treated with a suitable penicillin acylase.
Immobilized Alcaligenes faecalis penicillin G acylase is removed by filtration to give a clear solution.
The acylase activity is independent of the substituents at the 3-position of the cephem compounds.
The process of claim 7, wherein the deacylation is performed enzymatically using a dicarboxyl acylase.
The sequence of the complete acylase encoding region of plasmid pMcTGL7ANde was determined.
Each eluate was collected and lyophilized to obtain alpha ( beta ) chain of the acylase.
First, a number of selected acylase genes are cloned into a suitable expression host organism.
Enzymatic hydrolysis of the amide at the 7 - position of the cephalosporanic ring with glutaryl acylase.
Penicillin acylase enzymes, cephalosporin acylase and penicillin acyltransferase are examples of suitable heteromultimeric enzymes.
EDTA alone and FeCl3 enhance the acylase activity.
Introducing said mutant acylase gene into a host strain whereby a transformed host strain is obtained ;.
A process as claimed in claim 7 wherein the acylase enzyme is Savinase.
The assays for acylase activity are prepared in Eppendorf vials as follows,.
In addition Sizman et al . made various deletion mutants of penicillin acylase.
In case of amino acids the acylase requires the L-configuration at the Ca atom.
Regions of particular interest to mutate are the a-and B - subunits of the acylase.
The transcription regulating sequences of the penicillin G acylase gene as defined in claim 1 or 2.
The acylase concentration in the samples is calculated according to the following formula, EPMATHMARKEREP.
In this specification, a nomenclature for naming a specific mutant CC acylase is conveniently employed.
A mutant acylase according to claim 1, wherein said acylase is originated from Alcaligenes faecalis.
The translation regulating sequences of the penicillin G acylase gene as defined in claim 1 or 2.
A cephalosporin C acylase having the following characteristics,.
