Examples of 'affinity tags' in a sentence
Meaning of "affinity tags"
Affinity tags: Refers to markers or labels that indicate a close connection, relationship, or similarity between different items or categories
How to use "affinity tags" in a sentence
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affinity tags
The affinity tags allow easy purification.
Biologically produced cyclic affinity tags.
Such affinity tags are well known in the art.
The proteins may further comprise one or more affinity tags.
Two or more affinity tags may be used in combination.
Examples of groups that can be used to facilitate purification include polypeptides providing affinity tags.
Suitable affinity tags can also be artificially created.
Appropriate conditions for the dissociation of the different affinity tags are known in the art.
Affinity tags may also be referred to as fusión tags.
Such additional sequences and affinity tags are well known in the art.
Other affinity tags may also be used and are discussed below.
This Figure illustrates a pair of improved affinity tags derived from methionine.
A number of affinity tags and corresponding matrices are known in the art.
Ligands and ligand-binding moieties are paired affinity tags.
Nucleic acid affinity tags can be located within a nucleic acid.
See also
Non-limiting examples of suitable affinity tags are provided below.
Affinity tags are a convenient method of purification and immobilisation of recombinant proteins.
As discussed above the affinity tags described by Gygi et al.
Affinity tags can also be incorporated into the aptamer during preparation.
In some embodiments, the probe products are immobilized onto a substrate using their affinity tags.
A wide variety of affinity tags have been developed and are used throughout biotechnology.
Non-native can refer to affinity tags.
In addition to being affinity tags they provide resolution enabling tags of different sizes.
The heavy chain also carries hexa-histidine, c-myc and V5 affinity tags.
Such affinity tags may be used for the reversible attachment of the protein to a device.
An example of a useful affinity tags is the His6 tag.
Some of the affinity tags and reagents for their detection and isolation are available commercially.
Non-charged affinity tags can be used as well.
Such affinity tags are attached to the compound or to the polymerase by well-known methods.
Further affinity tags are well-known in the art.
The affinity tags can be added to the N-terminal or C-terminal end of the protein.
A plurality of affinity tags can be fused to a nucleic acid and / or a polypeptide sequence.
Some affinity tags have a dual role as a solubilization agent, such as MBP, and GST.
Nucleic acid affinity tags can be located 3 ′ to a nucleic acid.
Affinity tags are well-established tools for capturing a recombinant protein, e . g.
Pairs of affinity tags and predetermined targets are well-known to the skilled person.
Such affinity tags are attached to the probe by well-known methods.
Peptide affinity tags can be located N-terminal to a polypeptide sequence.
Peptide affinity tags can be located C-terminal to a polypeptide sequence.
Peptide affinity tags can be located N-terminal and C-terminal to a polypeptide sequence.
Examples of affinity tags include a six-histidine tag, trpE, glutathione and maltose-binding protein.
Preferred affinity tags according to the present invention are biotin, amylase, glutathione and poly ( His ).
Preferred affinity tags are biotin, amylase, glutathione and poly ( His ).
These affinity tags are described in " Chromatography-based Biomolecule Separations . ".
DNAs encoding affinity tags are available from commercial suppliers ( e.g., Pharmacia Biotech, Piscataway, NJ ).
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