Examples of 'alkaline lysis' in a sentence
Meaning of "alkaline lysis"
Alkaline lysis: Alkaline lysis is a technique commonly used in molecular biology to isolate plasmid DNA from bacterial cells by disrupting the cell membrane using an alkaline solution
How to use "alkaline lysis" in a sentence
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alkaline lysis
It is based on the alkaline lysis method.
Alkaline lysis methods were used for plasmid isolation.
Individual clones were used to prepare plasmids by alkaline lysis.
Plasmids were purified by alkaline lysis and ammonium acetate precipitation.
Alkaline lysis without a heat incubation step is significantly less efficient.
Plasmid was isolated by an alkaline lysis method.
The alkaline lysis and neutralization steps require a 5-minute incubation on ice.
Plasmids were extracted from the transformants by the alkaline lysis method.
Solubilisation of the enzyme by alkaline lysis was performed using a crude bacterial pellet.
Removal ofthe flocculent precipitate is yet another challenge in scaling up alkaline lysis.
The blocks were then prepared by standard alkaline lysis prep in a high throughput format.
These conditions were maintained for 6 h in order to achieve complete alkaline lysis.
DNA was extracted by the alkaline lysis method and treated with RNAse.
The plasmid present in this colony is extracted and isolated by the alkaline lysis technique.
Plasmid DNA was prepared by alkaline lysis and cesium chloride gradient centrifugation by standard methods.
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This has striking advantages during the subsequent alkaline lysis and purification.
DNA was isolated by alkaline lysis and PEG precipitation as above.
Plasmid DNA was isolated by the rapid alkaline lysis method.
For example, an alkaline lysis procedure may be utilized.
Plasmid DNA was prepared using a modified alkaline lysis procedure.
Hereby a standard alkaline lysis may be performed instead of using Qiagen-tip 20 a.
Plasmid DNA was isolated using a standard alkaline lysis miniprep protocol.
Figure 9 shows the result of the alkaline lysis procedure performed on an integrated cartridge.
The extraction of plasmid DNA was performed according to the alkaline lysis method.
RF DNA was recovered using the alkaline lysis method of Maniatis et al.
Individual colonies were selected and DNA was extracted using a alkaline lysis method.
Positive colonies were then screened by alkaline lysis mini-preps and BamHI restriction digestion.
A plasmid DNA was extracted from this transformant by the alkaline lysis method.
More concretely, the methods used are the alkaline lysis method and caesium chloride gradient purification.
Plasmid DNA from bacteria was isolated using the standard alkaline lysis method.
Total cosmid DNA was isolated by alkaline lysis procedure, and purified on CsCl gradient.
Thereafter, a target plasmid was obtained by alkaline lysis.
Plasmid DNA was prepared by the alkaline lysis method Maniatis et al, pp.
Plasmids were extracted from the thus-obtained transformants through the alkaline lysis method.
The pMP6-IL2 plasmids were purified by alkaline lysis and ammonium acetate precipitation.
PGenDel1 vector DNA was extracted from overnight cultures by the standard alkaline lysis procedure.
Plasmid was recovered by alkaline lysis and purified over an ion-exchange column ( Qiagen ).
Alternatively, plasmid DNA was isolated from 6 ml cell pellet by an alkaline lysis procedure.
Plasmids were purified using alkaline lysis procedures adopted and devised by Qiagen Corp. ( Chatsworth, CA ).
Double-stranded plasmid DNA templates were prepared by the alkaline lysis procedure.
M13 ds RF was isolated using the alkaline lysis method of Birnboim and Doily, Nucl.
Plasmid isolation from the transformants was carried out according to the alkaline lysis method [ 8 ].
DNA from identified clones was prepared by alkaline lysis miniprep DNA isolation J.
Plasmid isolation Plasmid isolation from E. coli was carried out according to the alkaline lysis method [ 13 ].
Recover RF DNA of mHSA140 using the alkaline lysis method of Maniatis.
Plasmids were purified from putative recombinants by alkaline lysis ( Maniatis et al . ).
Using centrifugation to remove cell debris Clarified lysate was produced by alkaline lysis in a 100g preparation.
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Examples of using Lysis
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Lysis from without could not be demonstrated
It may also lead to a lysis of certain particles
The lysis buffer comprises guanidine thiocyanate
Examples of using Alkaline
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Use high quality alkaline batteries only
Alkaline batteries into the battery compartment
When possible use alkaline battery replacements
