Examples of 'amidase' in a sentence
Meaning of "amidase"
Amidase (noun): An enzyme that catalyzes the hydrolysis of amide bonds in molecules
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- Any amidohydrolase
How to use "amidase" in a sentence
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amidase
Via the enzymatic pathway using an amidase.
This biological material expressing the amidase activity is preferably immobilized.
All compounds were effective inhibitors of anandamide amidase.
Expression of the enantioselective amidase in corynebacteria.
Also disclosed are novel compounds which inhibit anandamide amidase.
The cells expressing the glutaryl cephalosporin amidase were harvested by centrifugation.
Anandamide is cleaved in vivo by anandamide amidase.
A amidase capable of converting acephate or an analogue of acephate to its active metabolite methamidophos.
Ctrl was control where no amidase was added.
The enzymatic hydrolysis is preferably carried out in the presence of an amidase.
It is also possible that novel enantioselective amidase activities will be revealed.
In one embodiment the amidase enzyme is dried on the physiologically acceptable carrier.
The present invention provides substantially pure amidase enzymes.
Amidase catalyzes the hydrolysis of an amide to the corresponding acid or hydroxyl acid.
Under these conditions little significant denaturation of amidase takes place.
See also
The novel inhibitors of anandamide amidase disclosed herein also have research uses.
The present invention concerns polypeptides that possess an enantioselective amidase activity.
In this process the microbial cells containing amidase are separated from the fermentation broth.
It is then easy to verify that such genes code for an enantioselective amidase.
This document teaches the use of anandamide amidase inhibitors as analgesic agents.
This open reading frame represents the structural gene of the enantioselective amidase.
It is desirable that amidase enzymes having increased activity should become readily available commercially.
The inhibition of thrombin is demonstrated by in vitro inhibition of amidase activity of thrombin.
The amidase when heated by the method of the invention above exhibits very high activity.
The present invention is a method of inhibiting anandamide amidase in an individual or animal.
To do this, 3 nmol of the amidase preparation were reduced and carboxymethylated.
The present application describes a method of inhibiting anandamide amidase in an individual or animal.
The protein band of amidase 2 was indicated by an arrow bar.
This result indicates that palmitylfsulfonyl fluoride is highly effective at inhibiting anandamide amidase.
The reaction mixture was resolved by HPLC and the amidase activity was calculated.
An amidase domain as used herein preferably hydrolyzes gamma-glutamyl-containing substrates.
Such enzymes include the enzymes collectively referred to as penicillin amidase or penicillin acylase.
The protein band of amidase 2 is indicated by an arrow bar.
HSA possesses an esterase and amidase activity.
Preferably, the at least one amidase enzyme is formulated as a food or feed additive.
The cells were disrupted by sonication and the cell lysates assayed for penicillin G amidase activity.
This was done by subjecting 3 nmol of the amidase preparation to reduction and carboxymethylation.
The present invention concerns a DNA sequence coding for a polypeptide with enantioselective amidase activity.
Inhibition assays for formamidase and amidase 2.
Cell debris may then be removed by centrifugation leaving a crude cell-free extract containing amidase.
However, present inhibitors of anandamide amidase have disadvantages.
Measurement of the amidase activity The protocol is as follows,.
Periplasmic aggregation limits the proteolytic maturation of the Escherichia coli penicillin G amidase precursor polypeptide.
In further preferred embodiments, the amidase enzyme for use in the present invention is recombinant.
In this manner, it is possible to obtain high quantities of amidase in any microorganism.
It is found in a superfamily of amidases, including GSP amidase and peptidoglycan hydrolases.
Family member can include at least one, and preferably two amidase subdomains.
A study testing possible interactions with amidase inhibitors is part of the post-authorisation development plan.
The genes for lysing infected bacteria include the bacteriophage holin gene and / or an amidase gene.
Glycine and glycylamide also stimulated the amidase activity of each enzyme ( data not shown ).
