Examples of 'ammonium sulfate precipitation' in a sentence
Meaning of "ammonium sulfate precipitation"
ammonium sulfate precipitation: this phrase describes a chemical process where ammonium sulfate is used to cause the precipitation or separation of proteins or biomolecules from a solution. It is a technique commonly used in biochemistry and molecular biology for purifying and isolating proteins based on their differential solubility
How to use "ammonium sulfate precipitation" in a sentence
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ammonium sulfate precipitation
Cell sonicates were fractionated by ammonium sulfate precipitation.
Ammonium sulfate precipitation was the method chosen as a first step.
The concentrate was subjected to ammonium sulfate precipitation.
Ammonium sulfate precipitation is a common method for protein purification by precipitation.
The antibodies were partially purified by ammonium sulfate precipitation.
The ammonium sulfate precipitation is more effective than hydrophobic interaction chromatography.
The supernatant is then subjected to sequential ammonium sulfate precipitation.
Ammonium sulfate precipitation provides a convenient and simple means to fractionate complex protein mixtures.
The lysate is then fractionated with ammonium sulfate precipitation.
Ammonium sulfate precipitation and acid or chaotrope extraction may be used for fractionation of samples.
The resulting antiserum is treated with ammonium sulfate precipitation.
Ammonium sulfate precipitation is a first, and anionic exchange chromatography a second purification step.
The supernatant fluid was fractionated by ammonium sulfate precipitation as follows.
The ammonium sulfate precipitation removes additional impurities, and prepares the sCR1 for hydrophobic interaction chromatography.
The crude yeast extract was partially purified by ammonium sulfate precipitation.
See also
Purification For ICAM1 ammonium sulfate precipitation was first carried out.
Serum was collected and total IgG was isolated by ammonium sulfate precipitation.
Ammonium sulfate precipitation of culture media supernatant ( secreted protein ).
The IgG fraction was separated from serum by ammonium sulfate precipitation.
After proteins ammonium sulfate precipitation ( 0-90 % ) the proteins were separated using a chitin affinity chromatography column.
The results of this bioavailable testosterone test were obtained by ammonium sulfate precipitation.
IgG was purified from plasma by ammonium sulfate precipitation and protein G-Sepharose chromatography ( 27, 4 ).
Rabbit IgG was purified from preimmune or immune serum using conventional ammonium sulfate precipitation.
The recombinant product was concentrated by ammonium sulfate precipitation ( final concentration 70 % of saturation ).
The homogenate thus obtained is centrifuged, and the supernatant is fractionated by ammonium sulfate precipitation.
Fractions with activity were pooled, and ammonium sulfate precipitation was similarly performed.
All antibodies were obtained from serum-free hybridoma supernatants and purified by ammonium sulfate precipitation.
Immunoglobulin G also may be isolated by ammonium sulfate precipitation of blood serum.
Four to six liter batches of conditioned medium ( CM ) were subjected to ammonium sulfate precipitation.
Several other methods have been developed which employ ammonium sulfate precipitation as an early step.
The pI 7.0 fractions are pooled and the polybuffer ampholytes are removed by ammonium sulfate precipitation.
Fraction 45 was collected and concentrated by ammonium sulfate precipitation.
Fractions containing reductase activity were pooled and concentrated by ammonium sulfate precipitation ( 70 % saturation ).
The antibody was purified from the clear supernatant as follows, Step 1, ammonium sulfate precipitation.
Sevenoaks, Kent, GB further purified by ammonium sulfate precipitation.
The cell-free supernatant was subjected to a 50 % ammonium sulfate precipitation.
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