Examples of 'amplification step' in a sentence
Meaning of "amplification step"
The term 'amplification step' refers to a process or stage in which something is increased in magnitude, volume, or intensity. This phrase is commonly used in technical or scientific contexts to describe the act of boosting a signal or enhancing a particular quality
How to use "amplification step" in a sentence
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amplification step
The amplification step is performed with these primers.
Reagents for genetic amplification step.
The second amplification step is advantageously effected in gain saturation mode.
The third control is for the amplification step.
Additionally the amplification step may also introduce bias.
This can be followed by the complexity reduction or amplification step.
The method may comprise an amplification step as further explained below.
The quantification can or can not include an amplification step.
The amplification step substantially increases the sensitivity of the detection method.
Various primers can be used for the amplification step.
The amplification step and the hybridization step are preferably repeated at least once.
These methods generally require a reverse transcriptase step followed by an amplification step.
The cells obtained from the third amplification step were cloned by limiting dilution.
Such reverse transcription may be performed alone or in combination with an amplification step.
The addition of a chemilumigenic substrate during the amplification step allows for detection using luminescence.
See also
Substantially complicated and relatively large equipment is required to perform the amplification step.
The regenerator preferably further includes an amplification step before the shifting step.
Parallel amplification allows a high radio frequency power to be generated directly in a single amplification step.
In the case of RNA the amplification step is not necessary.
Any of a variety of commercially available kits may be used to perform the amplification step.
PCR amplification step was evaluated using electrophoresis agarose gel.
The analysis may be carried out during the amplification step of the method.
The amplification step is advantageously conducted using the PCR technique.
The methods of the technology described herein may further comprise an amplification step.
This initial amplification step normally takes about 3 weeks.
The primers used for the sequencing reaction were the same as for the amplification step.
In spite of all this, the amplification step is still required.
These artifacts often result from unequal use of diverse primers during the multiplexed amplification step.
The amplification step is optional and although desirable, is not essential.
Method for mapping a DNA molecule comprising an ad infinitum amplification step.
Typically, an amplification step is performed before proceeding with the genotyping.
DNA samples can be pooled or unpooled for the amplification step.
In each chamber, an amplification step can be performed using a different set of primers.
Designed for validation and quality control of the PCR amplification step.
Alternatively, the amplification step is ligase chain reaction or strand displacement amplification.
The TCR or immunoglobulin sequence can amplified using a primary and a secondary amplification step.
Figure 78 shows an embodiment useful when an amplification step is involved between selections.
It is advisable to remove primer B by purification prior to this amplification step.
Fig . 5 shows an embodiment useful when an amplification step is involved between selections.
In certain embodiments, a blocking oligonucleotide may be used in the amplification step.
In a preferred embodiment, the amplification step comprises one or more of the following,.
In certain embodiments, the detecting step involves an amplification step.
However, this amplification step is sensitive to contaminants which eventually lead to detection errors.
In some embodiments, the method does not comprise an amplification step.
In more specific aspects, an amplification step is performed after the hybridization and ligation steps.
In some aspects, the method does not comprise an amplification step.
Alternatively, the amplification step may be carried out in the presence of the scaffold / receptor complex.
In another aspect, the sample does not undergo a target amplification step.
Preferably, however, the amplification step is not conducted until after the second transformation described below.
Separating the extension products generated in the amplification step by size ;.
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