Examples of 'arginine deiminase' in a sentence
Meaning of "arginine deiminase"
Arginine deiminase is an enzyme that catalyzes the conversion of arginine to citrulline. It is commonly used in cancer therapy to deplete arginine, which some cancer cells require for growth
How to use "arginine deiminase" in a sentence
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arginine deiminase
It is an object of the present invention to provide a novel arginine deiminase.
The bacterial arginine deiminase expression could be regulated by various environmental factors.
The invention also relates to erythrocytes containing arginine deiminase.
The antitumor activity of arginine deiminase has been the subject of numerous publications.
Another object is to provide a method of manufacturing this novel arginine deiminase from mycoplasma.
Arginine deiminase transforms arginine in the presence of water into citrulline and ammonia.
Certain disadvantages have come to be associated with the isolation of arginine deiminase from organisms.
Arginine deiminase may be cloned from microorganisms of the genus Mycoplasma.
Such amino acid substitutions provide for improved renaturation of the modified arginine deiminase.
Chemical and genetic modification of the arginine deiminase enzyme can affect its biological activities.
The invention also concerns a strain of Lactobacillus brevis which is highly endowed with arginine deiminase.
In some embodiments the arginine deiminase is derived from a microorganism of the genus Mycoplasma.
The amino acid substitutions may thus provide for a modified arginine deiminase of the invention.
The production of arginine deiminase vÃa recombinant DNA techniques also provides for certain disadvantages.
Preferably the Mycoplasma hominis cell is deficient in arginine deiminase and thus requires complementation.
See also
The arginine deiminase preparations were chronically injected i . p.
Also discloses is a DNA sequence which encodes for the modified arginine deiminase of the invention.
The arginine deiminase of claim 1 convalently bound to polyethylene glycol.
In such systems, sequences that encode the modified arginine deiminase are typically located on a vector.
In particular, arginine deiminase can be covalently modified with polyethylene glycol.
In some embodiments of the invention, the modified arginine deiminase has multiple amino acid substitutions.
As a result, an arginine deiminase having the above-mentioned physicochemical properties is obtained.
Generally, increasing the molecular weight of the polyethylene glycol decreases the immunogenicity of arginine deiminase.
Accordingly, arginine deiminase must be properly formulated before being used as a therapeutic agent.
Example 2, Production of erythrocytes encapsulating arginine deiminase ml of blood is taken from the patient.
Most prokaryotic arginine deiminase pathways are under the control of a repressor gene, termed ArgR.
These problems may be overcome by covalently modifying arginine deiminase with polyethylene glycol ( PEG ).
Thus, arginine deiminase may be utilized as a treatment for melanomas, hepatomas and some sarcomas.
It has been shown the E. coli may produce large amounts of Mycoplasma arginine deiminase.
The location of lysine in arginine deiminase from such organisms may be indicated as follows,.
Figure 6B shows a similar alignment using ClustalW of P. aeruginosa DDAH and arginine deiminase.
Accordingly, when utilized, the arginine deiminase system may yield two molecules of ammonia.
The process of claim 17, further comprising recovering said expressed arginine deiminase.
Unfortunately, the formulation of arginine deiminase with polyethylene glycol often inactivates the enzyme.
In some embodiments from about 7 to about 15 polyethylene glycol molecules are bonded to arginine deiminase.
The arginine deiminase of the present invention is prepared from mycoplasma by the following method,.
Enzymes which degrade arginine are known and include arginine deiminase ( ADI ).
Preferably, arginine deiminase is cloned from microorganisms of the genus Mycoplasma.
Test substance ( ADILE ) consisted in arginine deiminase loaded red blood cells ( RBC ).
The arginine deiminase of claim 6 having an amino acid sequence comprising SEQ ID NO, 4.
Ii Substrate specificity The arginine deiminase hydrolyzes L-arginine to produce L-citrulline and ammonia.
The arginine deiminase of claim 1 comprising the amino acid sequence of SEQ ID NO, 2.
ERY-ADI is arginine deiminase ( ADI ) encapsulated in red blood cells.
In some embodiments arginine deiminase is derived from Mycoplasma hominis arginine deiminase ( SEQ ID NO, 1 ).
The P. aeruginosa arginine deiminase was also amplified using the oligonucleotides PaDEIM2 and PaDEIM3.
The modified arginine deiminase included glutamic acid at the 112 position and serine at the 210 position.
The isolated arginine deiminase of claim 15, having a specific activity of from about 3 to about 30 IU / mg.
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