Examples of 'arylsulfatase' in a sentence
Meaning of "arylsulfatase"
Arylsulfatase is a term used in biochemistry and molecular biology to describe an enzyme that catalyzes the hydrolysis of sulfate esters in arylsulfates, playing a crucial role in various metabolic processes
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- Any of a group of sulfatase enzymes.
How to use "arylsulfatase" in a sentence
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arylsulfatase
Enzyme preparations substantially free from arylsulfatase are described.
Arylsulfatase b deficiency rapidly progressing.
The elution of lactase partly overlaps with the elution of arylsulfatase.
Arylsulfatase is an enzyme that breaks down specific lipids called sulfate esters.
The polypeptide may be homologous or heterologous for said arylsulfatase deficient strain.
The arylsulfatase deficient strain is preferably produced according to a method as described herein.
Omission of these fractions is critical to prevent contamination of the lactase preparation with arylsulfatase.
The arylsulfatase deficient strain is preferably produced according to the described method.
The polypeptide may be any polypeptide whether native or heterologous to the arylsulfatase deficient strain.
The arylsulfatase deficient strain is preferably produced according to the method of the present invention.
Aryl sulfatase In another embodiment the polypeptide of interest may be an arylsulfatase A.
The arylsulfatase A may in particular be of human origin but not derived using human embryos.
MID is directly caused by a deficiency of the enzyme arylsulfatase A.
The arylsulfatase A may in particular be of human origin.
Process according to claim 1, wherein said lactase is heterologous to said arylsulfatase deficient strain.
See also
The human arylsulfatase A gene structure has been described.
The invention also provides, in further aspects, enzyme preparations substantially free from arylsulfatase.
Arylsulfatase A is an acidic glycoprotein with a low isoelectric point.
In one aspect, the disclosure features a method for purifying arylsulfatase A from a sample.
Recombinant arylsulfatase A for reducing galatosyl sulphatide levels in a subject.
In some embodiments, the method further includes subjecting the sample of arylsulfatase A to viral inactivation.
The fractions containing arylsulfatase A activity can be collected and combined for further purification.
The active substance in Naglazyme, galsulfase, is a copy of the human enzyme arylsulfatase B.
Clearly, levels of arylsulfatase activity are correlated with off-flavour formation.
A lactase is described which comprises less than 40 units arylsulfatase activity per NLU of lactase activity.
Arylsulfatase was measured in the extract using the method described in Example 2.
The buffer used for formulating the purified arylsulfatase A can include, for example, sodium chloride.
In some embodiments, the method further includes formulating the purified arylsulfatase A.
We have surprisingly found that arylsulfatase is a crucial enzyme activity, responsible for off-flavor formation.
Said first primer hybridizes to a first DNA strand of said arylsulfatase G gene ;.
In another embodiment, the arylsulfatase A is purified on a manufacturing scale.
In yet another embodiment, the method further includes formulating the purified arylsulfatase A.
Optionally filling the formulated preparation of recombinant arylsulfatase A into a suitable container and freeze-drying the sample.
This organic form containing principally ester S requires microbial mineralization to sulfate by arylsulfatase ( ARS ) enzyme.
In one embodiment, the arylsulfatase A is purified on a bench scale.
For example, the disclosure provides methods for purifying arylsulfatase A from a sample.
Finally, the arylsulfatase A can be eluted in an elution buffer.
A portion of the sequence in ( a ), which is enzymatically equivalent to recombinant human arylsulfatase A ; and.
Preferably, the arylsulfatase deficient strain is a K.
The disclosure relates, inter alia, to methods of producing arylsulfatase A.
In some embodiments, concentrating the arylsulfatase A includes tangential flow ultrafiltration.
Arylsulfatase A undergoes a pH-dependent polymerization forming a dimer at pH 4.5.
The BBB limits the delivery of arylsulfatase A ( ARSA ) from the blood to the brain parenchyma.
Arylsulfatase A catalyzes the reaction of a cerebroside 3-sulfate + H2O = a cerebroside + sulphate.
For example, an arylsulfatase A enzyme assay can be,.
Activity assay arylsulfatase, Arylsulfatase activity was determined using p-nitrophenylsulfate ( obtained from Sigma ) as a substrate.
However, “ ARSA ” may also refer to arylsulfatase A protein in some cases.
The ability of the arylsulfatase to release sulfate from intact 35S-PSGL-1 was examined.
As used herein, recombinant arylsulfatase A will be abbreviated “ rASA ”.
In the matured arylsulfatase A ( mASA ) the mentioned cysteine residue is Cys-51.
