Examples of 'assay buffer' in a sentence
Meaning of "assay buffer"
Assay buffer is a solution or mixture used in scientific experiments and tests to maintain the pH, ionic strength, and other properties necessary for the accuracy and reliability of the assay. It is specifically designed to optimize the conditions for the chemical reactions or measurements being performed
How to use "assay buffer" in a sentence
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assay buffer
The membranes were washed twice in assay buffer.
Assay buffer without enzyme is added to negative controls.
The monoclonal antibody is diluted in assay buffer.
The composition of the assay buffer is given below.
Prepare dilutions of compounds to be tested in assay buffer.
The specific assay buffer conditions are summarized as following.
Dilutions were made in the kit assay buffer.
A blank assay buffer was included.
The final pellet was suspended in assay buffer.
Zero standard consists of assay buffer containing detergent only.
Membranes were also diluted with this assay buffer.
Assay buffer and solutions were prepared on the morning of the assay.
All reagents are prepared in assay buffer.
Enzyme solution was prepared in assay buffer with a concentration specified below.
The pellets were washed twice with assay buffer.
See also
The assay buffer was saturated with air before the experiment.
Serial dilutions of test compound in assay buffer were added.
Assay buffer without Thapsigargin was used as a negative control.
Ul enzyme diluted in assay buffer is added.
Stock solutions of collagenase were diluted with the assay buffer.
Test compounds were prepared in assay buffer without probenecid.
The plasma membrane pellet was resuspended in cold assay buffer.
The buffer was used both as the assay buffer and incubation buffer for the sample.
Preferably the diluent is an assay buffer.
Fifty µl of Assay Buffer was added to each well prior to addition of sample.
There was no protein in the assay buffer.
The pellet was resuspended in assay buffer and briefly homogenized with a Polytron.
The substrate was made in assay buffer.
Fifty µl of assay buffer I is added to each well.
The dried extract is reconstituted in the assay buffer.
Negative controls consisted of assay buffer or PEI culture medium alone.
Cells were incubated overnight and washed once in assay buffer.
The Assay Buffer was then removed and the plate washed as before.
Preparation of the assay buffer.
The Assay Buffer was added to the Substrate Mix and mixed gently.
The final washed membrane pellet is resuspended in assay buffer.
The DELFIA Assay buffer and Enhancement solution were purchased from PerkinElmer.
The substrate wash solution was identical to the luciferase assay buffer.
The precipitated material was resuspended in assay buffer and was used for binding assay.
Enzyme solutions are prepared at the concentrations listed below in assay buffer.
The precipitate was rinsed with the assay buffer and recentrifuged.
Neutralisation of the extractant by including a neutralising agent in the assay buffer.
Beads were washed with Assay Buffer two times.
This incubation was terminated by washing twice in ice cold assay buffer.
The DELFIA Assay buffer and Enhancement solution can be purchased from PerkinElmer.
The scintillation proximity assay is carried out at room temperature in assay buffer.
An assay buffer of the following composition is prepared,.
Cell membrane fractions were prepared as previously described and resuspended in assay buffer.
Prepare 4x dilutions in assay buffer prior to use.
The charged cells were then washed and loaded with assay buffer.
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