Examples of 'auxotrophic' in a sentence
Meaning of "auxotrophic"
auxotrophic (adjective) - Referring to a microorganism that is unable to synthesize a particular compound required for its growth and reproduction, necessitating supplementation in its environment
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- Describing a strain of organism that requires a specific metabolic substance that the parent organism was able to synthesize by itself
How to use "auxotrophic" in a sentence
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auxotrophic
The double mutant is auxotrophic for methionine.
Both auxotrophic and prototrophic bacteria can be used.
Such a mutant organism is known as a auxotrophic mutant.
An auxotrophic strain or an antibiotic sensitive strain may also be used.
The resulting cell is then auxotrophic for uracil.
Auxotrophic for leucine.
A selection marker may confer antibiotic resistance or be an auxotrophic marker.
Auxotrophic heteroallelic strain.
Transformants resistant to the drug are auxotrophic for that nutrient.
This population is auxotrophic for methionine in the absence of sodium methylmercaptide.
The isolated spores are screened on the basis of their auxotrophic property.
Auxotrophic mutants useful as vaccine vectors may be generated in a number of ways.
A preferred method entails the inactivation of genes to create an auxotrophic phenotype.
Auxotrophic strains may be used in place of engineered tRNA and synthetase.
These modifications will result in strains which are auxotrophic for histidine.
See also
The amino acid auxotrophic prokaryotic cell is in one embodiment a bacterial cell.
Parental subpopulations of cells can be selected for known auxotrophic mutations.
Of an auxotrophic mutant.
Certain other embodiments expressly utilize only an auxotrophic host cell.
The said auxotrophic yeast is transformed with the above mentioned shuttle plasmid.
Parental subpopulations of cells can be selected with known auxotrophic mutations.
Cells with auxotrophic mutations are selected by replica plating on minimal and complete media.
The growth of these mutants may be auxotrophic to branched chain amino acids.
Those which grew on complete medium but not on minimal agar were auxotrophic.
Alternatively it is contemplated that the bacterium is auxotrophic for pantothenate when cured of a plasmid.
Markers will usually employ antibiotic resistance or impart prototrophy to an auxotrophic host.
Selection of a strain auxotrophic for thymine was carried out under the conditions recorded above.
This supplementation allows for the culturing of specific lines of auxotrophic recombinants.
A complementable auxotrophic mutation provides a selectable marker for use in transformation of the generated host.
The three diploid strains were also screened for auxotrophic nutritional requirements.
An auxotrophic expression system is known for Pseudomonas species.
Such propagation comprises growing the bacteria in media without the auxotrophic factor.
The prokaryotic cell can also be auxotrophic for more than one amino acid.
It is an auxotrophic strain generated by UV radiation and can not synthesize uracil.
Also listed is a minimal selection media based upon auxotrophic markers of the yeast strains.
This product contains a live auxotrophic deletion mutant Streptococcus equi vaccine strain with attenuated virulence.
The method also proved useful in the selection of additional auxotrophic mutations from single auxotrophs.
Generally, auxotrophic organisms are used for yeast.
This means that isoleucine may be present also when a strain is not auxotrophic for isoleucine.
The transformation of auxotrophic Neurospora crassa occurred with spheroplasts.
In some embodiments, the transformed bacterium is auxotrophic.
The recombinant Listeria strain may be an auxotrophic Listeria or a recombinant Listeria monocytogenes strain.
Auxotrophic markers were scored by replica plating to drop-out plates.
Further, it may contain an auxotrophic complementation marker.
Auxotrophic strains or antibiotic-sensitive strains also may be used.
Moreover, these cells will be auxotrophic for adenine.
Instead, an auxotrophic host strain will be complemented.
In this case, the strain remained auxotrophic for adenine.
Instead, an auxotrophic host strain is complemented.
In particular embodiments, the human cancer is an arginine auxotrophic cancer.
