Examples of 'bamhi site' in a sentence
Meaning of "bamhi site"
bamhi site - a term that may refer to a specific recognition site for the restriction enzyme BamHI in DNA sequences
How to use "bamhi site" in a sentence
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bamhi site
The BamHI site was filled in to create blunt ends.
Galt was cloned into the BamHI site of pCMV.
A single BamHI site was inserted after a strong staphylococcal promoter.
Oryza australiensis contained an additional BamHI site in the intergenic spacer.
Typically a BamHI site is included downstream of the coat sequence.
The back primer contains a BamHI site and a PstI site.
The unique BamHI site was inserted between the promoter and terminator fragments.
The larger one was the vector plasmid deleted from the BamHI site.
We also added a BamHI site after the internal SAlI site.
The cleavage sites of the last two enzymes are before the BamHI site.
A unique SalI site was inserted into the BamHI site using a synthetic oligonucleotide primer.
The nucleotide sequence is numbered from the first nucleotide shown see BamHI site of Fig.
Both plasmids have a unique BamHI site between the promoter and the terminator.
Contains the TAKA promoter and AMG terminator and has a unique BamHI site as a cloning site.
Simultaneously a unique BamHI site was introduced in lieu of the FKB I coding sequences.
See also
Before transcription, plasmids were linearized at the BamHI site.
Example 4 is inserted at the BamHI site of the said transfer vector.
This a-region is controlled by the λPr promotor inserted at a BamHI site.
An artificial BamHI site was introduced flanking the 5 ' end of the coding sequence.
A termination codon is inserted at that position, following a BamHI site.
A mutant which had the introduced BamHI site was selected and designated pBHI-bam.
Oligo m · carried a silent point mutation which generated a BamHI site.
The distance between the BamHI site and the beginning of transcription was only 2 bases.
The sense primer contained an EcoRI site and the antisense, a BamHI site.
The purified BgIII fragment was cloned into the BamHI site of the expression vector pET9a.
The sites designated B° correspond to the point of a ligation between a BglII site and a BamHI site.
The GATA-2 specific primer contained a BamHI site to facilitate subsequent cloning.
The PCR product was digested with BamHI and inserted into pCMV-Neo-Bam at the BamHI site.
The mutagenesis created a BamHI site in the upstream region of the Tox21a sequence.
Oligonucleotide 1, Designed to prime just before the BamHI site in the pki promoter.
A convenient BamHI site is located downstream to nucleotide -7.
A ‘ stuffer ' is present between NcoI and BamHI site.
Oligonucleotide 6, Designed to put a BamHI site at the end of the pyruvate kinase terminator.
The 3 ' end also was changed by removing an internal BamHI site.
The L1 gene was inserted into the BamHI site of the baculovirus transfer vector.
One silent situation is also indicated ( * ) which created a unique BamHI site.
Note that the 3 ′ BamHI site should be destroyed.
The tyrosine hydroxylase gene fragment was cloned into the BamHI site of p184DLARG.
There was also a new BamHI site introduced at the 3 ' end of the neo gene.
This fragment is ligated into the BamHI site of pUC 19.
The 5 ′ primer included a BamHI site and the 3 ′ primer included an XhoI site for cloning purposes.
The plasmids were screened for the appropriate orientation of the 2086 gene in the BamHI site.
This fragment is cloned at the BamHI site of vector pQE30.
A BamHI site is located at the 45th codon of the COBS gene.
Now, to continue the construction, the BamHI site has to be eliminated.
Introduction of a BamHI site one nucleotide 3 ' to the TAG termination codon.
The 3.2 kb BstNI fragment containing epiB was inserted into the BamHI site by blunt-end ligation.
Introduction of a BamHI site seven nucleotides 5 ' to the AUG methionine initiation codon ;.
PCR products were cloned into the HindIII and BamHI site of the pNeoBg140.
In Table 14 the BamHI site is italicized and the termination codon is underlined.
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Examples of using Bamhi
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This fragment was digested by BamHI and ligated
The BamHI restriction site is represented by a black diamond
The volume was increased and BamHI added
