Examples of 'bglii' in a sentence
Meaning of "bglii"
bglii - abbreviation for a restriction enzyme used in molecular biology
How to use "bglii" in a sentence
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bglii
They all carry a BglII restriction site.
A BglII site is included at the junction of the deleted sequence.
This new vector was digested with BglII and EcoRI.
A XhoI and a BglII sites were introduced with the primers.
The products were then digested with HindIII and BglII.
This fragment comprises the BglII and HindIII cohesive ends.
This adapter fragment was cleaved by the enzymes NcoI and BglII.
The underlined BglII site is added for plasmid construction purposes.
The new restrictive enzymes used were BglII and KpnI.
The plasmid in which BamHI and BglII sites disappeared was named pMSCVNAAB.
The new plasmid was next cleaved with BglII and NcoI.
For BglII and NcoI only sites relevant for the construction are included.
Orientation of the fragment was checked with BglII and EcoRI enzymes.
The BglII site is filled by Klenow enzyme.
The modified fragment is inserted into the BglII site of pXBG.
See also
All oligonucleotides are introducing BglII restriction sites in frame of the rex coding sequence.
Then this ligation mixture was digested with AvrII and BglII.
The ligation reaction was possible because BglII and BamHI ends are compatible with each other.
It was then cut with the compatible enzymes BglII and SalI.
The BglII to EcoRI fragment of Bbk was created in several steps.
The correctly mutated plasmid was identified by its BglII and BstYI site changes.
This plasmid has a unique BglII site available for the final construction step see Fig.
A recombinant hpAP minigene was designed and inserted into the BglII site of pAd.
The plasmid is linearized with BglII and amplified by means of PCR.
The right construct can be isolated and checked by a restriction digest with BglII.
BglII to generate the complementing plasmid.
These asymmetrically phosphorylated adapters also contain an internal BglII restriction enzyme site.
BglII is a type II restriction endonuclease isolated from certain strains of Bacillus globigii.
For the modification of the ends for example HindIII and BgLII can be used.
The boldface type denotes a BglII site tail to convert the 5 ' boundary to a BglII site.
The resultant product was then digested with the restriction endonucleases EcoRI and BglII.
The DNA is digested with BglII and EcoRI.
Partial deletions of this construct were made using the internal restriction sites EcoRI and BglII.
Following amplification the DNA is digested with BglII and treated with Klenow.
The resulting DNA product was digested with the restriction enzymes SacII and BglII.
Cutting of the plasmid pEA1 with the enzyme BglII gives several fragments.
The PCR product was purified by phenol extraction and digested with Nsil and BglII.
Subsequently, a BglII sub-clone was used for sequence analysis to obtain more sequence data.
The DNA was digested with either BglII or PstI.
A unique BglII site is positioned near the 5 ' end of the inserted heavy chain encoding sequence.
Both PCR fragments were digested with BglII and ligated.
Any BglII resistant sequences were then cloned, sequenced, and compared with the known sequences.
The PCR product was digested with BamHI and BglII and cleaned.
BglII to generate the complementing plasmid, pAdCBhpAP.
The PCR was then digested with restriction enzyme BglII and NheI.
Therefore, expression of any insert at the BglII and BamHI sites would generate a nonfused peptide.
The product of the PCR is purified and digested with the AscI and BglII enzymes.
A unique BglII site is positioned near the 5 ' end of the inserted Fc encoding sequence.
Examples of restriction enzymes include HindIII, DpnII and BglII.
This cassette is introduced into the BglII site downstream of the left T-DNA border.
