Examples of 'binding buffer' in a sentence
Meaning of "binding buffer"
A binding buffer is a solution or substance used in certain laboratory techniques or procedures to facilitate the attachment or bonding of molecules or particles. It helps to create favorable conditions for interactions and promotes the formation of stable complexes or associations between different components
How to use "binding buffer" in a sentence
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binding buffer
Discard the binding buffer using a pipette.
A solution of was prepared of a mixture of peptides in binding buffer.
In binding buffer was added to each well.
Ml syringe was filled with binding buffer.
Membranes were washed in binding buffer and visualized by autoradiography.
The compounds are serially diluted into binding buffer.
In preferred embodiments the binding buffer does not include alcohol.
The filters were washed with five changes of binding buffer.
Four hundred microliters of binding buffer was then added to each sample.
The plate was then washed twice in binding buffer.
The gel is washed with binding buffer and elution buffer.
Plates are washed three times with binding buffer.
Binding buffer was discarded and samples and standards were added to the wells.
Cells were resuspended in binding buffer.
The binding buffer can be warmed briefly before use to resuspend the urea.
See also
After completion of the kinase reaction a binding buffer is added.
The binding buffer will generally contain high salt and polyalkylene glycol concentrations.
The columns were then washed repeatedly with binding buffer.
The cells were then washed twice in binding buffer before analysis by flow cytometry.
Free ligand was removed by repeated centrifugation and resuspension in binding buffer.
Membranes and radioligand are diluted in binding buffer without protease inhibitors.
The sample solution was adjusted to the composition of the binding buffer.
The chips then were washed twice with binding buffer to remove unbound proteins.
The column was regenerated offline by washing with a large volume of binding buffer.
The cells were then washed with binding buffer and analyzed by flow cytometry.
The beads were washed three times with the binding buffer.
The binding buffer was removed and the beads were washed four times with wash buffer.
The positive control is made by adding binding buffer without competitor.
The described binding buffer with slight modifications can also be used as the wash buffer.
Preferably the equilibration buffer and the binding buffer are identical.
The binding buffer and DNA were mixed well vÃa vortexing.
Unbound ligand was removed by by three washes with ice cold binding buffer.
Chips were then washed three times with binding buffer and then twice with water.
The media was removed and cells were washed twice with cold binding buffer.
The beads are washed in binding buffer and used to capture biotinylated DNA.
The filter is washed three times with ice cold binding buffer.
Wash was repeated with SureSelect Binding Buffer two more times for a total of three washes.
Cells were placed on ice and washed twice with cold binding buffer.
The DNA concentrator uses a DNA binding buffer which may induce lysis of the bacterial cells.
The agarose beads are then precipitated and washed four times with binding buffer.
The cells may then be washed twice with binding buffer and resuspended in DPBS.
The medium was then aspirated and the cells were washed three times with binding buffer.
The binding buffer had the following composition,.
The cells are concentrated and lysed with the lysis binding buffer supplied with the kit.
Remove the binding buffer and add the following to the well types indicated,.
An aliquot of each cell pellet is washed in PBS and resuspended in a binding buffer.
For this purpose, 5 ml of binding buffer are spread over the column in each case.
The DNA was then bound to cellulose particles in presence of the binding buffer.
First, the magnetic beads are washed in a binding buffer to remove storage and preservative solution.
The peptide analog is dissolved in PBS and serially diluted in binding buffer.
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Examples of using Binding
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Compliance with legally binding regional protocols
Legally binding provisions on availability of unleaded fuel
Adopt rigorous and binding reporting rules
Examples of using Buffer
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No buffer zones are defined for the site
The boundary of the buffer zone is adequate
The buffer has had too much
