Examples of 'biotinylated antibody' in a sentence
Meaning of "biotinylated antibody"
Biotinylated antibody: An antibody that has been chemically linked to biotin, a vitamin that is often used as a tag for detection in biological experiments, such as immunohistochemistry or flow cytometry
How to use "biotinylated antibody" in a sentence
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biotinylated antibody
Unbound biotinylated antibody was removed by washing the wells.
Chemibeads were added to bind excess free biotinylated antibody.
A biotinylated antibody would then be detectable by avidin or streptavidin binding.
Slides were washed again and a secondary biotinylated antibody was added.
Reactivity of the biotinylated antibody was also examined against other normal tissues.
Chemibeads were added to scavenge the excess free biotinylated antibody.
The order of addition of biotinylated antibody and standards is not critical to the procedure.
The streptavidin is diluted in the same way as the biotinylated antibody.
The results indicated that radiolabeled biotinylated antibody was stable in vitro at all time points.
Sensibeads were then added to bind to the biotin portion of the biotinylated antibody.
A matrix approach was used where each biotinylated antibody was tested against each cold competitor.
Sensibeads are then added and bind to the biotin portion of the biotinylated antibody.
Binding of biotinylated antibody decreases with increasing amounts of chimeric and control murine antibodies.
In one preferred embodiment the haptenylated antibody is a biotinylated antibody or a digoxigenylated antibody.
The biotinylated antibody was then buffer exchanged into PBS.
See also
Preferably, the detecting antibody is a biotinylated antibody.
The biotinylated antibody was purchased from Jackson Immunoresearch Laboratories.
In a preferred embodiment, the detection agent is a biotinylated antibody.
Preparation of biotinylated antibody against monoclonal anti-AB antibody.
In one embodiment, the detectable antibody is biotinylated antibody.
The biotinylated antibody was then coupled to blue latex as follows,.
The method of claim 1 wherein said capturable component is a biotinylated antibody.
Ul of diluted serum and of biotinylated antibody are mixed together in a well, in duplicate.
The device of claim 11 wherein said capturable component is a biotinylated antibody.
The biotinylated antibody is in turn reacted with streptavidin-horseradish peroxidase complex.
The streptavidin-HRP is diluted in the same way as the biotinylated antibody.
Alternatively, the biotinylated antibody is combined with other tumor specific antibodies.
After several washes to remove unbound proteins, a secondary biotinylated antibody is added to the reaction.
For example, a biotinylated antibody can be assayed using an avidin-peroxidase conjugate and an appropriate substrate.
After PBS wash, flow cytometric analysis was carried out using a biotinylated antibody.
The phage was pre-incubated with the biotinylated antibody at room temperature for 1 hour.
Biotinylated antibody 4B8 was preincubated with mononuclear cells from cord blood.
Incubation of the slides with the biotinylated antibody was performed at room temperature for 2 hours.
Most preferably each tube is coated with approximately 250 ng of biotinylated antibody.
Cells which reacted with the biotinylated antibody were subsequently reacted with PE-labeled strepavidin.
The beads are streptavidine-coated, to which a biotinylated antibody will bind.
A method for removing biotinylated antibody from a cell surface without substantially affecting the cell surface, comprising,.
Only data obtained with 1 µg / mL of biotinylated antibody is shown.
They were then stained with biotinylated antibody specific for transferrin receptor, followed by Strepavidin-Cyt3 ( PharMingen ).
Captured anti-Pneumovax IgM were revealed using an anti-IgM biotinylated antibody and neutravidin-conjugated HRP.
The biotinylated antibody is stored at 4°C until ready for use.
The reaction mixture was then incubated with biotinylated antibody to form a 25 ( OH ) D-biotinylated antibody complex.
The biotinylated antibody 17 is then bound to the polystreptavidin layer 16.
As illustrated in Figure 1C, binding compounds comprise a biotinylated antibody ( 140 ) as a binding moiety.
The bound biotinylated antibody is detected with the 125I-streptavidin.
The intra-assay and inter-assay precision was < 9 % . Preparation of the biotinylated antibody.
For detection biotinylated antibody Goat 1 was used.
The binding capacity of the beads was approximately 8.0 µ g biotinylated antibody per mg beads . Example 41.
Briefly, the biotinylated antibody was digested enzymatically with 1 % protease at 37°C for four hours.
For this purpose, the biotinylated antibody MCA1753 is added to the microtitration plates for 1 hour.
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Examples of using Biotinylated
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The fraction containing biotinylated IgG are collected
Biotinylated oligonucleotides are now ready for use
The fraction containing biotinylated IgG were collected
Examples of using Antibody
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Antibody status should be monitored and reported
The kinetics of antibody decline are also similar
Antibody persistence after booster immunisation adults
