Examples of 'blocking buffer' in a sentence
Meaning of "blocking buffer"
blocking buffer: In biology and chemistry, a blocking buffer is a solution used to prevent nonspecific binding of reagents or antibodies to a surface. It helps to block or inhibit unwanted interactions, thereby enhancing the specificity and accuracy of experimental results
How to use "blocking buffer" in a sentence
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blocking buffer
Blocking buffer alone is used as a positive control.
The antibodies were diluted in the blocking buffer.
Blocking buffer is removed and compound mixture pipetted.
All of the antibodies were diluted in blocking buffer.
Blocking buffer was used as a negative control.
Blots were incubated with antibodies in blocking buffer.
Test proteins in blocking buffer were added at various concentrations.
Standards and serum samples were diluted in blocking buffer.
Dilute serum in blocking buffer and add to membrane.
Standards were diluted in blocking buffer.
The blocking buffer was discarded by inversion and manual shaking.
Dilute conjugate in blocking buffer and add to membrane.
The blocking buffer was removed before addition of the tested sample.
Comparable thrombin concentrations in blocking buffer were used as reference.
The blocking buffer is removed by washing the beads on a sintered glass filter.
See also
Washing twice with and storage in blocking buffer was then done.
The blocking buffer was removed and replaced with same buffer containing primary antibody.
Antibodies were diluted in blocking buffer.
Correlation between blocking buffer intensity values and average intensity.
The membrane is blocked for one hour with blocking buffer.
Correlation between blocking buffer intensity and average intensity within rows.
The controls are produced by incubating the slides in the presence of blocking buffer.
Remove the blocking buffer and wash the plate just before adding lysate.
The nitrocellulose was then washed three times in excess volumes of antibody blocking buffer.
Aspirate the blocking buffer by vacuum filtration and wash two times with flush buffer.
Following transfer the membrane was immersed for one hour with gelatin blocking buffer.
Filter through the blocking buffer using a Millipore filtration system.
Proteins were transferred onto PVDF membranes and saturated in blocking buffer.
Blocking buffer was removed and plates were rinsed twice more with PBS.
Selection conditions were as described below with BSA replacing milk in the blocking buffer.
Discard any Blocking buffer remaining at the top or bottom of the concentrator.
The protein was transferred to nylon membrane and blocked with Western blocking buffer.
The blocking buffer is filtered through using a Millipore filtration system and then toweled dry.
Enzyme molecule denaturation on the glass surface was prevented by blocking with a BSA blocking buffer.
Place membrane in blocking buffer for 2 hours.
Blocking buffer is added to the HGF-only control wells.
Each membrane is immersed in a Petri dish containing blocking buffer kept stirring for one hour.
A blocking buffer is added and reacted at room temperature for 5 minutes.
Test sera are diluted in PBST blocking buffer.
Following blocking, the blocking buffer is removed from the wells.
Non-specific binding sites were blocked by incubation with a blocking buffer.
Place membrane in blocking buffer for 2 hr.
The cells were then pelleted and resuspended in 1 ml of cold blocking buffer.
Substrate was blocked with blocking buffer for 2 hours.
The strips were treated for 2 h with monoclonal antibodies diluted in blocking buffer.
Determine the volume of blocking buffer needed,.
Prepare the appropriate dilution of secondary antibody-alkaline phosphatase conjugate in blocking buffer.
After overnight incubation, blocking buffer was aspirated from plates.
The cells were then incubated with primary antibody overnight at 4oC in blocking buffer.
The kit of claim 8, further comprising a blocking buffer and an incubation buffer.
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No buffer zones are defined for the site
The boundary of the buffer zone is adequate
The buffer has had too much
Examples of using Blocking
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The floating switch is blocking in automatic operation
Blocking direct sunlight during cooling opera
The silt is blocking their port hatch
