Examples of 'bluescript' in a sentence
Meaning of "bluescript"
bluescript: Describing a type of plasmid vector used in genetic engineering, often associated with the study of genes
How to use "bluescript" in a sentence
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bluescript
Unique cDNA clones are purified and subcloned into bluescript vector.
Bluescript phagemid inserts were excised from positive clones for further analysis.
A fragment of the recombinant phage which contains the cDNA is subcloned into the bluescript plasmid.
Bluescript plasmid derivatives were excised from recombinant λ phages as for cDNA clones.
Applicants have modified the Bluescript plasmid by inserting a tyrosinase gene.
Bluescript plasmids containing these DNA inserts were then sequenced.
The Sanger method was used to sequence fragments of interest inserted in a Bluescript plasmid vector.
The Bluescript plasmid has been modified by inserting a tyrosinase gene.
A suitable vector is the Bluescript vector of Stratagene.
Six positive clones are isolated, purified and subcloned into the bluescript plasmid.
The PCR products were subcloned into the Bluescript vector to confirm the nucleotide sequence.
In a related series of experiments, the cDNAs were cloned into the bacterial vector bluescript.
The product was subcloned into the Bluescript KS plasmid and sequenced.
Bluescript plasmids are recovered by infecting an F ′ strain and plating on Ampicillin plates.
The resulting products were subcloned into the Bluescript KS plasmid and sequenced.
See also
Two subclones of this Bluescript vector are created by site-directed mutagenesis as follows,.
The in vivo excision protocol ( Stratagene ) was carried out to liberate Bluescript phagemids.
The amplified fragment was subcloned into Bluescript KS plasmid and subjected to DNA sequence analysis.
The transposon is constructed in E. coli in a BlueScript vector.
The plasmid vector Bluescript SK+ has been found to be suitable.
Transfer of the IL-li cDNA from a lambda phage to a Bluescript plasmid cloning vector.
The inserts were ligated to a Bluescript plasmid vector and E. coli TG1 competent bacteria were transformed.
The purified cDNA fragments were cloned into the Bluescript plasmid ( Stratagene ).
Bluescript vector ( pBS ) was used as negative control.
This product was then cloned into the plasmid Bluescript KS available from Stratagene.
The Bluescript plasmid DNA ( 10 ng ) was used as a positive control for the transformation.
According to the instructions provided by Stratagene, a BlueScript plasmid was rescued from the phage vector.
DNA Sequencing -- Restriction fragments of the rat genomic clones were subcloned into Bluescript plasmid.
These clones were inserted into a Bluescript KS-vector using Eco RI as described above.
The PCR products were gel purified, restricted and ligated into Bluescript for sequencing.
Bluescript series vectors ( Stratagene ), were used.
According to the present invention, the commercially available Bluescript SK vector was employed.
After tertiary screening, a positive cDNA clone was subcloned into Bluescript ( Stratagene ).
The pUC19 plasmid backbone was derived from a Bluescript SK ( + ) vector with an ampicillin resistance gene.
Phagemids were released vía co-infection with helper phage and recircularized to generate Bluescript SK - ( Stratagene ).
Attempts to clone the entire cDNA into the E. coli Bluescript plasmid initially failed.
Reaction 2, The DNA template was the LO-CD2a VH region in Bluescript.
The insert of pBS3-15 was determined to be a Bluescript vector fragment contaminant.
Reaction 2, The DNA template was the LO-CD2a VI clone in Bluescript.
The λ cam H6a cDNA was subcloned into the plasmid Bluescript KS for sequence analysis.
The HindIII / NotI fragment was recloned into the bluescript KS+ vector.
The obtained PCR fragment was subcloned into the Bluescript SKII ( - ) plasmid ( Stratagene ).
Following second strand synthesis, the cDNA was inserted into the bacterial Bluescript plasmid ( Stratagene ).
The cells are tested for efficiency using 1 pg of a Bluescript plasmid ( Stratagene ).
An XbaI / PstI fragment containing part of intron 1 was inserted into Bluescript SK ( Stratagene ).
The 5 ' and 3 ' RACE products were subcloned into the plasmid Bluescript KS and sequenced.
The immunoglobulin gene inserts were excised and sub-cloned into Bluescript ( Stratagene, USA ).
The DNA was ligated into appropriate cut restriction sites of Bluescript vector ( Stratagene, CA ).
Sequencing of cDNA inserts The EcoRI cDNA inserts are subcloned into Bluescript KS ⁺ Vector ( Stratagene ).
PMCN-2a was a correctly-deleted intron-less mutant in the Bluescript phagemid.
