Examples of 'blunt-ended' in a sentence
Meaning of "blunt-ended"
blunt-ended (noun) - in genetics and molecular biology, a term used to describe DNA fragments that have ends lacking overhanging nucleotides. Blunt-ended fragments are often generated by certain restriction enzymes and are utilized in various molecular cloning techniques
How to use "blunt-ended" in a sentence
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blunt-ended
The nasal bones were short and blunt-ended.
A blunt-ended probe is then used to initiate the next cycle.
Both the vector and insert were blunt-ended and ligated.
The heavy blunt-ended lines represent portions used as probes.
Alternatively the complex may be blunt-ended at one or both ends.
The resulting blunt-ended fragment was gel purified by standard methods.
Furthermore, digestion of the vector with HpaI results in a blunt-ended fragment.
These siRNAs can be blunt-ended or can have overlapping ends.
In other embodiments disclosed herein, one or both of the strands are blunt-ended.
The digested, blunt-ended plasmid was recircularized in ligation conditions as described above.
Another alternative embodiment of the present invention provides blunt-ended molecules.
Individual cells had blunt-ended nuclei with evenly distributed nuclear chromatin.
Another alternate aspect of the present disclosure provides blunt-ended molecules.
The ligation may be a blunt-ended ligation or using a protruding single stranded sequence e . g.
The samples were cooled on ice and the cDNA was blunt-ended as follows.
See also
The blunt-ended linear fragments were ligated to the adapters provided by the manufacturer.
Double stranded molecules may be blunt-ended or have single strand overhanging ends.
This blunt-ended duplex was capable of making concatamers upon multiple ligation events.
In other embodiments of the present disclosure, one or both of the strands are blunt-ended.
The fragments in the digest mixture were blunt-ended and ligated with EcoRI linkers.
The blunt-ended fragments can be phosphorylated by enzymatic treatment, for example using polynucleotide kinase.
The SacI restriction is thus effected in order to produce the desired blunt-ended SacI-HindIII fragment.
Restriction fragments can be blunt-ended or have protruding ends, depending on the endonuclease used.
The combination of these activities therefore generates blunt-ended DNA segments.
Cleavage can result in blunt-ended or staggered cleavage products dependent upon the restriction enzyme employed.
The presence of such cohesive termini is generally preferred over blunt-ended DNA molecules.
The present invention relates to a blunt-ended cannula device for use with a pre-slit resealable injection site.
Prior to digestion with SstIId, the cohesive HindIII ends is made blunt-ended using Klenow fragment.
The two fragments were ligated to a blunt-ended SphI / EcoRI fragment containing the yeast TPI promoter.
The blunt-ended linear molecules were then cleaved with Bam HI and purified.
This DNA fragment is rendered blunt-ended by treatment with mung bean nuclease.
The digested plasmid was treated with Klenow enzyme to create blunt-ended vector DNA.
The two fragments were ligated to a blunt-ended Sphl / EcoRI fragment containing the yeast TPI promoter.
The blunt-ended fragment was then phosphorylated using the T4 polynucleotide kinase in the presence of ATP.
Both of these enzymes generate blunt-ended DNA fragments.
The two preceding blunt-ended fragments are ligated together and transformed into competent E. coli.
This procedure fills in the protruding ends of the Cla I cut to leave a blunt-ended fragment.
The SalI cohesive end was made blunt-ended using the large fragment of DNA polymerase I.
The result is a population of cDNAs, which are blunt-ended.
The plasmid pACMAB was blunt-ended by digestion with Sal I.
In other embodiments, one or both of the strands are blunt-ended.
The fragment was made blunt-ended by T4 DNA polymerase according to standard procedures.
The other strand is blunt-ended.
This blunt-ended fragment is then ligated into pCG-1 prepared above.
The protruding Bglll and EcoRI ends are made blunt-ended by the polymerase I Klenow fragment.
In another embodiment, both ends of the siRNA are blunt-ended.
Fractionating the blunt-ended cDNA so formed ;.
In another embodiment, one end of the siRNA is blunt-ended.
Are ligated with the blunt-ended double-stranded cDNAs.
The double-stranded antisense compounds may be blunt-ended siRNAs.
