Examples of 'by in vitro transcription' in a sentence
Meaning of "by in vitro transcription"
by in vitro transcription - Process of synthesizing RNA molecules in a controlled environment outside a living organism, typically in a test tube or a cell-free system. It involves using biochemical tools and enzymes to create RNA strands from a DNA template
How to use "by in vitro transcription" in a sentence
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by in vitro transcription
M indicates a marker prepared by in vitro transcription of a synthetic template.
Given are the designation and the sequence of mRNAs obtained by in vitro transcription.
Wherein the RNA is obtained by in vitro transcription or chemical synthesis.
The final aptamer sequence identified can then be prepared chemically or by in vitro transcription.
The control RNA spikes were produced by in vitro transcription of linearized plasmid templates.
The dsRNA molecules useful for the invention may also be produced by in vitro transcription.
MRNA may be generated by in vitro transcription from a DNA template.
A radioactive antisense RNA probe is synthesized by in vitro transcription.
RNA may be obtained by in vitro transcription of an appropriate DNA template.
The polypeptide may be provided from the nucleic acid by in vitro transcription and translation.
RNA fragments produced by in vitro transcription were purified using RNeasy MinElute Cleanup Kit ( Qiagen ).
Several approaches were described for increasing the production of RNA molecules by in vitro transcription.
The antisense probe was synthesized by in vitro transcription from a PCR product.
CRNA, Single-stranded RNA complementary to a DNA, synthesized from the latter by in vitro transcription.
RNA transcription RNA was generated by in vitro transcription of the PCR product.
See also
Full length RNA transcripts were prepared from the above cDNA clones by in vitro transcription.
Preferably, mRNA is produced by in vitro transcription using a DNA template.
In one embodiment, the synthetic RNA molecule is produced by in vitro transcription.
Recombinant TF may be obtained by in vitro transcription and translation, for example.
Single-stranded RNA oligonucleotide can be obtained by in vitro transcription.
Linearization followed by in vitro transcription using T7 RNA polymerase produced cRNA transcripts.
CTG leucine Generation of Coxsackie virus stocks by in vitro transcription and transfection.
The RNA is produced by in vitro transcription using a polymerase chain reaction ( PCR ) - generated template.
The new spacer encoding CRISPR RNA was obtained by In vitro transcription.
The probes were labeled by in vitro transcription using 35S-labeled UTP.
Secondly, chemically modified mRNA was produced by in vitro transcription.
The RNA test mixture was prepared by in vitro transcription from a 110 base single-stranded DNA template.
In one embodiment, the RNA has been obtained by in vitro transcription.
The oligoribonucleotides were produced by in vitro transcription using the transcription kit " AmpliScribe T7 " ( Epicentre Technologies ).
According to the invention, mRNA may be prepared from a DNA template by in vitro transcription.
Double-stranded RNA was prepared by in vitro transcription using the T7 RiboMAX ™ Express RNAi System ( Promega ).
A RNA molecule useful with the invention can conveniently be prepared by in vitro transcription ( IVT ).
Wherein polyribonucleotides are produced by in vitro transcription or chemical synthesis.
FIGS . 1 to 3 show sequences encoding mRNAs that can be obtained by in vitro transcription.
Step ( E ) refers to labeling by in vitro transcription.
A ribonucleic acid ( RNA ) molecule can be produced by in vitro transcription.
MRNA obtained from this vector accordingly by in vitro transcription is designated as " PpLuc ( GC ) - A64 ".
The self-replicating RNA can conveniently be prepared by in vitro transcription ( IVT ).
The replicon can conveniently be prepared by in vitro transcription ( IVT ).
This demonstrates the synthesis of the sc A6 TCR-C-Kappa TCR by In vitro transcription translation.
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