Examples of 'cas protein' in a sentence
Meaning of "cas protein"
cas protein - CAS proteins are a class of proteins that play various roles in gene regulation and immunity, particularly in prokaryotic organisms
How to use "cas protein" in a sentence
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cas protein
The Cas protein may be linked to a protein transduction domain.
A comprehensive review of the Cas protein family is presented by Haft et al.
A Cas protein can be fused to a heterologous polypeptide that provides for subcellular localization.
More than forty different Cas protein families have been described.
The Cas protein derived from Streptococcus pyogens may recognizes NGG trinucleotide.
The invention further comprehends the Cas protein being codon optimized for expression in aEukaryotic cell.
The protein-binding segment of a subject gRNA interacts with a Cas protein.
The guide RNA and the Cas protein may function as a pair.
The Cas protein of the first system or the second system is a Cas3 protein or.
In some methods, the first Cas protein is a nickase.
A solution of a Cas protein along with a section of guide RNA begins the CRISPR process.
Alternatively, a modified or engineered Cas protein can be employed.
In some embodiments, the Cas protein is codon-optimized for expression in a eukaryotic cell.
In one embodiment, the first nucleic acid sequence encodes a human codon-optimized Cas protein.
In an embodiment of the invention the Cas protein is a type II CRISPR-Cas protein.
See also
Likewise, a Cas protein and a gRNA can be introduced into the call as a protein-RNA complex.
Class 2 CRISPR systems use a single Cas protein with a crRNA.
The Cas protein cuts invading phage 's DNA to stop infection.
In one embodiment, the Cas protein is a type II Cas protein.
The Cas protein can cleave the first CRISPR RNA recognition sequence to generate a double-strand break.
In some embodiments, the Cas protein is a type II CRISPR system enzyme.
A “ gRNA-Cas complex ” includes a complex of a Cas protein with a gRNA.
The Cas protein can be fused to a heterologous polypeptide, such as a nuclear localization signal ( NLS ).
In one embodiment, the Cas protein is a type I Cas protein.
In some embodiments, the NIS is located at the N-terminus of the cas protein.
Preferably, Cas protein is Cas9 protein or variants thereof.
Formation of crRNAs, processing of pre-cr-RNAs producing of mature crRNAs to guide the Cas protein.
In some embodiments, the Cas protein is a Cas9 protein.
In other embodiments, the NIS is located the C-terminus of the cas protein.
In some methods, the Cas protein is a Cas9 protein.
In particular embodiments, the Cas protein is Cas9.
For example, the Cas protein can be a Cas9 protein or be derived from a Cas9 protein.
In specific embodiments, the Cas protein is Cas9.
The Cas protein can be Cas9.
In some such embodiments, the Cas protein is Cas9.
The Cas protein is a Cas9 enzyme.
In some such methods, the Cas protein is Cas9.
For example, the Cas protein can be a Cas9 protein.
In some of the above methods, the Cas protein is Cas9.
In some instances, a Cas protein is from a type II CRISPR / Cas system.
In one embodiment, the type II Cas protein is Cas9.
Optionally, the Cas protein is Cas9.
The method of embodiment 1, wherein the Cas protein is Cas9.
In one embodiment, the Cas protein is Cas9.
In some methods, the first Cas protein is Cas9.
The kit of claim 7, wherein the cas protein is cas9.
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