Examples of 'cation exchange column' in a sentence
Meaning of "cation exchange column"
cation exchange column - This phrase refers to a chromatographic column used in ion exchange chromatography, specifically designed for separating and analyzing cations based on their charge properties. It is commonly employed in biochemistry, analytical chemistry, and molecular biology to isolate and purify cationic molecules within a chemical mixture
How to use "cation exchange column" in a sentence
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cation exchange column
The conjugate was purified on a cation exchange column.
The pool of the cation exchange column was concentrated.
Reaction products are loaded on a cation exchange column.
The cation exchange column is valved into the system.
Asa was purified on a cation exchange column.
A suitable cation exchange column is a column whose stationary phase comprises anionic groups.
The contaminating proteins in the starting material were retained on the cation exchange column.
The ion exchange column is cation exchange column or anion exchange column.
Ion exchange column used according to the present invention is a cation exchange column.
The supernatant was purified by cation exchange column chromatography to obtain a partially purified sample.
Optionally the ion exchange column is an anion exchange column or a cation exchange column.
The choline was separated on a cation exchange column coupled with suppressed conductivity detection.
Filtered LGG culture supernatant was loaded onto a cation exchange column.
The eluted HAO is applied to a cation exchange column in essentially the same buffer.
The Protein A eluate can then be further purified using a cation exchange column.
See also
cation exchange capacity
cation exchange chromatography
cation exchange resin
cation exchange resins
cation exchange chromatography
cation exchange resin
cation exchange resins
The cation exchange column is eluted with sodium chloride in a pH 5 sodium acetate buffered solution.
Excellent purification has been obtained using a Mono S cation exchange column.
Loading the diafiltered sample on a cation exchange column for purification in bind and elute mode ;.
That is, at a particular pH and ionic strength we equilibrated a cation exchange column.
The solution emerging from the cation exchange column as effluent separated into 3 separate fractions.
In certain aspects, the ion exchange column is a cation exchange column.
The method of E1, wherein said second cation exchange column comprises a carboxymethylated or sulfonated matrix.
The ionic strength should be below 8mS/cm before application to the cation exchange column.
Prior to HIC, the eluate from the cation exchange column was diluted with ammonium sulfate.
A method according to Claim 1 wherein the cation exchange chromatography uses Fractogel ® in a cation exchange column.
In this optional third step, a cation exchange column is used.
The cation exchange column can be a POROS ® 20HS cation exchange resin from Applied Biosystems.
For the quantitative determination of saccharides, a cation exchange column is used.
An S Sepharose ( Pharmacia ) cation exchange column may also be employed as a final buffer exchange step.
Eluting purified non-complexed botulinum toxin A from the cation exchange column.
Purifying the mature viral protease using a cation exchange column comprising Fractogel ®.
This mixture was poured over a Varian Mega Bond Elut ™ strong cation exchange column.
Applying said diluted centrifugation supernatant to a cation exchange column equilibrated at about pH 7.5 ;.
It is then filtered through a 0.45 micron filter prior to loading onto a cation exchange column.
Eluting PAF-AH enzyme from said cation exchange column using 1M salt ;.
The method of claim 14, wherein the ion exchange chromatography comprises a cation exchange column.
A second purification step was performed by means of a cation exchange column HiPrep 16/10 SP FF.
IV-1 suspension before loading onto the strong cation exchange column.
FIG . 10 illustrates the manner of loading of broth after diafiltration on cation exchange column.
Eluting purified non-complexed botulinum toxin A from the cation exchange column of step ( d ).
FIGS . 2A-D depict variations in purification runs on a cation exchange column.
Mono S ( Pharmacia ) is especially suitable to the strong cation exchange column.
After the pepsin digestion, the GAH antibody F ( ab ' ) 2 was purified using cation exchange column chromatography.
The crude heterodimer sample was loaded on a Poly CAT A cation exchange column at 20ml/min.
Following concentration, IL-21 is captured on a cation exchange column.
Purifying the IL-21 refolded protein on a cation exchange column.
FIG . 11 illustrates the manner of loading of broth, after heat treatment, on cation exchange column.
The dialyzed sample was then loaded onto a 20ml SP Sepharose ™ cation exchange column ( GE Healthcare ).
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