Examples of 'cdna probe' in a sentence
Meaning of "cdna probe"
cdna probe: Refers to a single-stranded DNA molecule that is complementary to a specific messenger RNA (mRNA) sequence. It is used in molecular biology and genetics to hybridize and detect the presence of the corresponding mRNA in a sample. cDNA probes are valuable tools in research for studying gene expression, detection of specific RNA molecules, and molecular cloning
How to use "cdna probe" in a sentence
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cdna probe
Ubiquitin cDNA probe as loading control.
Several sequences that hybridized with the OvIFNt cDNA probe were identified.
The generated cDNA probe was used for hybridizing to cDNA microarrays.
The smaller message is not detected with the cDNA probe at higher stringency.
Infected bacteria are plated and submitted to a second round of screening with the cDNA probe.
The signal obtained with a rat actin cDNA probe is shown for comparison.
The genomic clone was identified in this library by hybridization with the dextranase cDNA probe.
This library may be screened with an oligonucleotide or cDNA probe obtained as described before.
Multiple human fetal and adult tissue northern blots were probes with a human fused cDNA probe.
Radioactively labelled cDNA probe to TH was hybridized to the filter and detected by autoradiography.
Those clones were selected that hybridize to only the cDNA probe from the induced culture.
The cDNA probe used is shown as a solid bar below the cDNA scheme in Fig.
Control hybridization with a GAPDH cDNA probe is shown in the lower panel.
A cDNA isolate was identified that appeared to hybridize weakly with the FPR cDNA probe.
A fetal kidney cDNA library was screened with a 5 ' cDNA probe and two hybridizing clones were identified.
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These results indicate the presence of human DNA sequences complementary to the OvIFNt cDNA probe.
Once again, a control hybridization with a normal beta-actin cDNA probe is shown below the hybridizations.
The results showed that the amplified sequences hybridized with the HCV cDNA probe.
The obtained cDNA probe was purified by using ProbeQuant ( produced by Amersham Pharmacia ).
The cellular RNA was hybridised with a cDNA probe of mouse Slugh.
This analysis employed a cDNA probe derived from the OvIFNt cDNA sequences ( Example 9B ).
Two mRNA species hybridized to the a2 subunit cDNA probe.
FISH detection was carried out with a Mus81 cDNA probe labelled with biotinylated dATP.
Northern blot analysis using hTERT-specific cDNA probe.
SPA was mapped through RFLP using a cDNA probe and a specific single nucleotide polymorphism ( SNP ) marker.
The membranes were hybridized with a fluorescein-labeled cDNA probe.
The rat B-integrin cDNA probe was a kind gift from Staffan Johansson, Uppsala, Sweden.
The filter was hybridised with a rabbit beta-globin cDNA probe.
Similarly, cDNA probe 2 was prepared from amplification product 2.
A depicts a Northern blot hybridization with LuFad3 cDNA probe.
The radioactive labelled cDNA probe has a specific activity of 5x10 ⁸ dpm / µg.
The filter was then rehybridized with a murine B-actin cDNA probe.
Transcript levels were corrected for RNA loading by using a cDNA probe for the U1 small ribonucleoprotein.
Tetracycline-resistant clones are screened for inserts by colony hybridization, using poliovirus cDNA probe M.
Rehybridization is performed with a GAPDH cDNA probe ( lower panel ).
The mRNA was then transferred to membranes and probed with radioactive CYP2A6 cDNA probe.
The blots were re-probed with a B-actin cDNA probe for control.
The Southern blots are probed with human EPOa-hSA fusión protein cDNA probe.
After transferring the bands to a Nylon membrane, hybridization with a DIG-labeled cDNA probe was performed.
Expression of PC-LECTIN was confirmed by northern blotting using a PC-LECTIN cDNA probe.
Preferably, the probe is a cDNA probe.
Metaphase showing FISH with the biotinylated DPP8 cDNA probe.
The charge was controlled, re-treating the filters with a cDNA probe of B-actin.
Approximately 1 x 106 clones from the human placenta cDNA library were screened with a bovine cDNA probe.
The blot was hybridized with the TB15 cDNA probe.
Northern blot analysis was performed using a full length E2F-1 cDNA probe.
Ethidium bromide stained gel . b blot hybridized with a LAG-3 specific cDNA probe.
This subgenomic library was plated and hybridized with an OvIFNt cDNA probe ( Example 7A ).
Three genomic clones in lambda DASHII which hybridized to the 5126 cDNA probe were isolated.
The gel is then Southern blotted and probed with the 700 bp cDNA probe.
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The cDNA is divided into two portions
The orientation of bikunin cDNA was confirmed by sequencing
A cDNA was also isolated from a human library
