Examples of 'cdna synthesis' in a sentence
Meaning of "cdna synthesis"
cdna synthesis - The process of creating complementary DNA (cDNA) from messenger RNA (mRNA) templates, commonly used in molecular biology for various applications
How to use "cdna synthesis" in a sentence
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cdna synthesis
Enriched fractions are used for cDNA synthesis.
The cDNA synthesis was stopped by rapid cooling on ice.
Compositions and methods for cDNA synthesis.
Commercially available cDNA synthesis kits can be utilized in the synthesis.
Different primers may be used for cDNA synthesis.
Second strand cDNA synthesis may then proceed using conventional techniques.
Improved methods for cdna synthesis.
This prevents cDNA synthesis by reverse transcription from continuing in the pooled samples.
The first step in the assay is cDNA synthesis.
The corresponding cDNA synthesis protocoll was adapted towards optimization of cloning into the pGBFin vectors.
The internal standard was added to the sample prior to cDNA synthesis.
Each laboratory performed cDNA synthesis and TaqMan analysis.
A special oligodeoxynucleotide is used for priming of first strand cDNA synthesis.
The reactions were carried out under standard cDNA synthesis methods given in the commercial kit.
Three pentadecamers are preferred for use as either probes or as primers for cDNA synthesis.
See also
Optimization of cDNA synthesis.
Synthesizing first strand cDNA from mRNA by employing conventionally available first strand cDNA synthesis kit.
RNA extraction and cDNA synthesis were performed using standard procedures.
The synthesis can be carried out by using a commercially available cDNA synthesis kit.
Three µl of the cDNA synthesis reaction was used in the subsequent PCR amplifications.
The total rna was extracted and purified for subsequent cdna synthesis and qpcr analysis.
The cDNA synthesis was performed with the cDNA synthesis kit of Pharmacia.
The total RNA is used to direct cDNA synthesis.
RNA preparations cDNA synthesis and cloning.
Samples were then processed for total RNA and cDNA synthesis.
Thermocycling can induce first strand cDNA synthesis from the RNA transcript target.
MoMLV reverse transcriptase is used for first strand cDNA synthesis.
Extraction of complete RNA and the following cDNA synthesis were performed as described above.
Second strand cDNA synthesis was performed by the method of Gubler and Hoffman.
The first strand cDNA was synthesized by following the BRL cDNA synthesis protocol.
Second strand cDNA synthesis was performed with RNAse H treatment followed by random priming.
The ferrochelatase primers and PCR fragment represent a control demonstrating that cDNA synthesis had occurred.
For cDNA synthesis the Strategene cDNA Synthesis KIT was used.
Schematic representation of cDNA synthesis and PCR reactions.
First and second strand syntheses were performed using a Gibco BRL cDNA synthesis kit.
First-strand cDNA synthesis was performed with a cDNA synthesis kit.
RNA extraction and cDNA synthesis.
A typical kit for cDNA synthesis contains the following components,.
CDNA was synthesized using a BMB cDNA synthesis kit.
Preferably, the first strand cDNA synthesis is synthesised by a reverse transcriptase.
The reverse transcription reaction was performed using the RevertAid First Strand cDNA Synthesis kit.
Second strand cDNA synthesis was performed according to the manufacturer 's instructions.
RNA isolation and cDNA synthesis.
Double-strand cDNA synthesis using random primer is performed by known methods.
Extraction of mRNA and cDNA Synthesis.
A number of cDNA synthesis protocols have been described which provide substantially pure full-length cDNA libraries.
RNA preparation and cDNA synthesis.
For the above-mentioned cDNA synthesis, a commercially available cDNA synthesis kit may also be used.
Total RNA from the tissue was isolated and cDNA obtained using a cDNA Synthesis System.
The protocol consists of two parts, cDNA synthesis and paired-end library preparation.
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The cDNA is divided into two portions
The orientation of bikunin cDNA was confirmed by sequencing
A cDNA was also isolated from a human library
Examples of using Synthesis
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Compilation and synthesis of fourth national communications
Two other documents complete this synthesis
Third synthesis report on activities implemented jointly
