Examples of 'cell culture supernatant' in a sentence
Meaning of "cell culture supernatant"
cell culture supernatant: The liquid that lies above a layer of cells growing in a culture dish and includes secreted products from the cells. This phrase is commonly used in the context of laboratory research, particularly in the fields of cell biology, microbiology, and biotechnology
How to use "cell culture supernatant" in a sentence
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cell culture supernatant
Allantoic fluid or cell culture supernatant.
The cell culture supernatant is used for further experiments.
Harvesting the cell culture supernatant.
In one specific embodiment the culture supernatant is a mammalian cell culture supernatant.
Fifty µl of cell culture supernatant was added.
Production of immunoglobulin from the cell culture supernatant.
The feed was cell culture supernatant adjusted to different loading buffer conditions.
Said sample may also stem from a cell culture supernatant.
Cell culture supernatant containing the recombinant virus was harvested and stored in aliquots.
It can be a cell culture supernatant.
The biological sample may suitably comprise cell culture supernatant.
Depleted cell culture supernatant with unrelated IgM antibodies served as negative control.
Their catalytic properties and stability in cell culture supernatant were determined.
Optionally the cell culture supernatant is prepurifed vía a Protein A column.
Purifying the antibody mixture produced in step c from cell culture supernatant.
See also
Concentration of the protein in the cell culture supernatant was calculated using standard curve.
The starting solution preferably comprises mammalian or insect cell culture supernatant.
It is rapidly released into the cell culture supernatant when the plasma membrane is damaged.
Afterwards they are incubated with the monoclonal antibody in the cell culture supernatant.
It is rapidly released into the cell culture supernatant upon damage of the plasma membrane.
And wherein said first or said second product stream is a cell culture supernatant.
Antibodies were purified from cell culture supernatant by protein A chromatography.
Also disclosed are compositions containing inactivated rotavirus produced directly from cell culture supernatant.
The control in these studies was depleted cell culture supernatant at a similar concentration.
Recovering said expressed polydonal protein from the cell culture cells or cell culture supernatant.
The antibody is purified from cell culture supernatant on a protein A sepharose column.
The sample may be a tissue sample obtained from the mammal or a cell culture supernatant.
The cell culture supernatant was prepurifed vía standard Protein A affinity chromatography.
Propagated whole virus is released into said cell culture supernatant from which it is collected.
The cell culture supernatant and cell remains were collected after 7 days.
They can be concentrated in large quantity from the cell culture supernatant by established methods.
The cell culture supernatant was applied to the column with a flow rate of 4 ml / min.
The method of any preceding claim wherein the cell culture supernatant is buffered with bicarbonate.
After thermal inactivation, the inactivated rotavirus may be isolated from the cell culture supernatant.
In various examples, the cell culture supernatant may be filtered prior to heating.
The results are presented in pg / ml in the cell culture supernatant.
After heating, the cell culture supernatant may be transferred to a new vessel.
Preparation of rhG-CSF containing cell culture supernatant.
Fifty ul of cell culture supernatant was added followed by a 1-2 hour incubation.
Afterwards, the coated biosensors were blocked in mock cell culture supernatant.
Proteins were purified from the cell culture supernatant vía Protein-A and size exclusion chromatography.
Optionally, the reagent can be added directly to the cell culture supernatant.
Three days post-infection, the cell culture supernatant was assayed for rAAV vector particles.
Moreover, a pharmaceutically acceptable adjuvant may be administered with the cell culture supernatant.
By purifying the polypeptide present in the cell culture supernatant by using chromatographic methods e . g.
Recovering the T5 or T8 capsular polysaccharide from the inactivated cell culture supernatant.
Fifty µl of cell culture supernatant was added followed by a 1 - 2 hour incubation.
Be cell extracts for intracellular proteins, or cell culture supernatant for secreted proteins.
Cell culture supernatant 2.
Recombinant human a-extended fibrinogen was purified from cell culture supernatant according to standard methods.
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