Examples of 'cell lysate' in a sentence
Meaning of "cell lysate"
Cell lysate refers to the liquid solution obtained after cells are broken open, releasing their contents. It is commonly used in various laboratory techniques and experiments to study the components and properties of cells. The cell lysate contains a mixture of proteins, nucleic acids, enzymes, metabolites, and other cellular molecules. Researchers often isolate and analyze specific molecules or biological activities from the cell lysate for further investigation or manipulation
How to use "cell lysate" in a sentence
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cell lysate
Cell lysate was applied onto the column.
The supernatant fluid is the total cell lysate.
The diluted cell lysate was stored on ice.
A biotinylated bait molecule is mixed with a cell lysate.
The above cell lysate was prepared as follows.
Here the mixture is a cell lysate.
A cell lysate was prepared by vortexing with glass beads.
Purifying the polypeptide from the cell lysate.
The cell lysate was then added to the supernatant.
Viral growth was evaluated in supernatant and cell lysate.
The cell lysate can originate from a cell.
A sample pool can be prepared from a cell lysate.
Three cell lysate samples were prepared.
The addition of precanavalin to a cell lysate is likewise preferred.
The cell lysate is spun down in a cold room.
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The separated and gained supernatant was used as cell lysate.
The cell lysate can be a lysate of prokaryotic or eukaryotic cells.
The proteins were obtained from the total cell lysate.
The cell lysate is then evaluated by counting in a scintillation counter.
The fluorescence of the cell lysate was measured as described above.
Cell lysate and conditioned medium were normalized by cell number.
Subjecting the clarified cell lysate to a hydrophobic interaction membrane.
Cell lysate addition method.
A nucleic acid sample can originate from cell lysate.
Samples of cell lysate and elution showed strong fluorescence.
This process is developed to purify adenovirus from cell lysate.
The cell lysate is centrifuged and the supernatant discarded.
The induced protein was in the cell lysate insoluble pellet.
Partially or completely free of other polypeptides or part of a cell lysate.
The tumor cell lysates is a prostate tumor cell lysate.
This invention relates to a vessel for mixing a cell lysate.
The cell lysate may also be clarified by the following procedure.
The sample may also be a tissue extract or a cell lysate.
Both cell lysate and culture fluid fractions were then analyzed.
Yields are expressed as a percentage of total protein in the cell lysate.
Suitable methods for producing cell lysate are known in the art.
Yields were expressed as a percentage of total protein in the cell lysate.
The cell lysate may be obtained following lysis of a cell paste.
The method of the invention may be applied directly to a cell lysate.
Vpr followed by a cell lysate will yield a bimodal elution curve.
The process of the invention can be applied directly to a cell lysate.
The cell lysate is flocculated by adding cationic polyelectrolytes and recentrifuged.
The cells can be ruptured and be in the form of a cell lysate.
The solution or cell lysate may be centrifuged without a sucrose gradient.
The mixed population of nucleic acids can be present in a cell lysate.
Unbound cell lysate is then removed by washing with wash buffer.
Dilution of cell lysate.
Cell lysate is prepared by any technique commonly employed in the art.
The reaction mixture is prepared using a cell lysate or cell extract.
The pH of the cell lysate can be reduced to form a stabilized cell lysate.
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Examples of using Lysate
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The clear lysate was deposited on the column
A preferred sample according to the invention is a lysate
Production of a crude lysate possessing enzymatic activity
