Examples of 'cell monolayer' in a sentence
Meaning of "cell monolayer"
cell monolayer: In biology, a cell monolayer refers to a single, continuous layer of cells that are attached and grown on a flat surface, such as the bottom of a Petri dish
How to use "cell monolayer" in a sentence
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cell monolayer
Nearly complete destruction of the cell monolayer.
The cell monolayer was lifted by gentle mechanical agitation.
Incubate at room temperature until cell monolayer detaches.
The formation of a cell monolayer was verified by an inverted microscopic analysis.
Unattached virus is removed by washing the cell monolayer.
Confluent cell monolayer culture.
The specimen volume should be sufficient to cover the cell monolayer.
Complete detachment and disaggregation of the cell monolayer is confirmed by microscopy examination.
The experiment firstly involves the development of confluent cell monolayer.
They demonstrated that the cell monolayer is indeed functional as a living membrane.
Wells were inspected microscopically for integrity of the red cell monolayer.
Plaques were picked from the cell monolayer and their progeny were further propagated.
These assays generally involve growing a confluent cell monolayer first.
The integrity of the cell monolayer can be monitored at this stage by microscopic examination.
This is particularly preferred once a closed endothelial cell monolayer has been formed.
See also
The remaining cell monolayer is assayed for cell viability by MTS staining.
Each culture well will be selected which contains a confluent cell monolayer.
Positive plaques are picked from the cell monolayer and their progeny are replated.
The plate is examined for adherence or lack of adherence to the astrocytoma cell monolayer.
The medium is removed and the cell monolayer is washed once with DPBS.
A viral plaque is formed when a virus infects a cell within the fixed cell monolayer.
HESC are plated as a single cell monolayer without the use of a stromal feeder layer.
One approach was to follow transport of a radioactive labelled compound across the epithelial cell monolayer.
The present invention also provides compositions comprising a cell monolayer produced according to these methods.
The fixed cell monolayer is incubated with antisera against either poliovirus proteins or S antigen.
The flask is periodically examined microscopically until dissociation of the cell monolayer can be confirmed.
The cell monolayer is rinsed twice with DMEM.
Studies have also shown that Fucans inhibit endothelial cell monolayer binding.
The cell monolayer was lifted by gentle mechanical agitation and trituration with a 25 mL pipette.
Instead, continuous endothelia are characterized by an uninterrupted endothelial cell monolayer.
In particular, a quiescent endothelial cell monolayer can serve as a barrier to infection, inflammation, and calcification.
A sodium fluorescein solution was then applied to each cell monolayer for 30 minutes.
The Caco-2 cell monolayer is an in vitro tissue culture model of the intestinal epithelium.
By 3 hours after protease addition, zones of clearing occurred in the cell monolayer.
The serum containing medium was removed and the cell monolayer washed twice with serum free-medium.
This protocol was used to measure the influx of compounds across a CaCo-2 cell monolayer.
As a control, HBSS was applied to a cell monolayer in place of the test solutions.
The radioactive media was removed by aspiration, with care not to disturb the cell monolayer.
Evaluation Criteria, Response of the cell monolayer was evaluated either visually or under a microscope.
The three circular vector DNAs were precipitated with CaPO4 and added to the cell monolayer.
The integrity of the cell monolayer is checked by measuring the transepithelial electrical resistance ( TEER ).
DNA / IMDM solution gently poured into flask so as not to disturb cell monolayer.
The integrity of the cell monolayer was checked by measuring the transepithelial electrical resistance ( TEER ).
Then, the media was removed and the replicon cell monolayer was washed once with PBS.
The cell monolayer was lifted with 2 mM EDTA in PBS.
The medium was then removed by aspiration and the cell monolayer washed with serum-free DMEM.
A cell monolayer was formed after 21 days in culture.
Remove the Opti-MEM I in the cell monolayer.
The cell monolayer was stained with a 0.4 % Trypan Blue solution and then inspected under a microscope.
Toxicity scores of 2, 3 or 4 are considered a high level or toxicity to the cell monolayer.
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