Examples of 'cell-free extract' in a sentence
Meaning of "cell-free extract"
Cell-free extract: It refers to the solution obtained after breaking open cells and removing cell debris, leaving behind soluble cell components. This extract is commonly used in biochemistry research to study cellular processes outside of a living cell environment
How to use "cell-free extract" in a sentence
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cell-free extract
Cell-free extract was prepared as described above.
The recovered eluate was the crude cell-free extract.
Cell-free extract of each strain was prepared as follows.
The supernatant thus obtained was used as a cell-free extract.
The cell-free extract was used as a sample for the activity measurement.
The resulting supernatant was used as the cell-free extract.
The cell-free extract is then treated as described above for whole cells.
Any method can be used to produce a cell-free extract.
The cell-free extract is prepared from the harvested microbial cells by using an ordinary method.
The cell debris was removed from the disrupt by centrifugation to give a cell-free extract.
The CR enzyme activity of the cell-free extract was measured in the following manner.
On the other hand, most of the peptides tested were hydrolyzed by the cell-free extract.
The BRD activity of this cell-free extract was determined in the following manner.
Cell debris may then be removed by centrifugation leaving a crude cell-free extract containing amidase.
Initially, a crude cell-free extract is prepared with harvested whole cells of Streptomyces clavuligerus.
See also
The thus-treated solution was centrifuged to remove insoluble fractions and prepare a cell-free extract.
The aminopeptidase was purified from a crude cell-free extract of Lactococcus lactis subsp.
Preparation of cell-free extract Cell-free extract was prepared from the obtained bacterial cell suspension.
The supernatant fraction so obtained is designated the cell-free extract ( cfe ).
Also provided is an Arabidopsis thaliana cell-free extract comprising the enzyme of the invention.
The cell-free extract ( crude enzyme solution ) was prepared as follows.
The CRD enzyme activity of the cell-free extract was measured in the following manner.
The disrupted microorganisms were centrifuged to remove cell debris, thereby obtaining 2880 ml of a cell-free extract.
Thus a cell-free extract was prepared.
From the obtained bacterial cell suspension, 1 ml of cell-free extract was prepared as follows.
The crude cell-free extract was applied to a DEAE-Sephacel ® column.
This diluted sample is designated as cell-free extract ( CFE ).
Preferably, a eukaryotic cell-free extract is used as an expression system.
Fig . 2 shows the results of DEAE-Sephacel ® column chromatography of a crude cell-free extract of S . cremoris Wg2.
Preferably a cell-free extract is made.
The crude cell-free extract was placed on a DEAE-Sephacel column.
Protamine sulfate was added to the cell-free extract to a final concentration of 0.5 %.
The preparation of cell-free extract was conducted similarly to Example 11.
Ammonium sulfate was added to the cell-free extract so that 30 % saturation was achieved.
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Studies with cell-free enzyme preparations
Cell-free extract was prepared as described above
The method is performed in a cell-free environment
