Examples of 'chemical ligation' in a sentence
Meaning of "chemical ligation"
chemical ligation: Chemical ligation is a term used in biochemistry and molecular biology to describe the joining of two molecules through a chemical reaction. It involves the formation of a covalent bond between components, often leading to the creation of a larger compound or complex
How to use "chemical ligation" in a sentence
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chemical ligation
Various possibilities for chemical ligation exist.
The chemical ligation was incubated at room temperature overnight.
Assembly of polypeptide building blocks by chemical ligation.
Native chemical ligation provides precisely that capability.
One aspect of the invention is directed to a method of native chemical ligation.
Means can be chemical ligation means or enzymatic ligation means.
Current strategies for lipopeptide synthesis also consist in using chemical ligation.
The chemical ligation process can optionally include addition of a tailpiece.
It therefore opens the door for in situ chemical ligation in living systems.
Chemical ligation methods have not been applied to methods of sequence analysis.
Several model peptides have been synthesized by the method of native chemical ligation.
Chemical ligation and amplification can also be used to join binding regions.
The preferred method of attachment employs a combination of peptide synthesis and chemical ligation.
Chemical ligation is a set of techniques used for creating long peptide or protein chains.
In some embodiments an adaptor comprises a functional moiety for chemical ligation to a nucleotide analog.
See also
Native chemical ligation relies on the presence of a cysteine residue at the ligation site.
Longer peptides could be made by conjoining individual synthetic peptides using native chemical ligation.
The method of native chemical ligation facilitates the chemical synthesis of proteins and large oligopeptide.
Model studies were undertaken with small peptides to investigate the native chemical ligation approach.
Chemical ligation can result in non-covalent as well as covalent bonds.
The most frequently used technique for synthesizing proteins is Native chemical ligation NCL.
In further embodiments, chemical ligation includes a splint oligonucleotide in the binding reaction.
The conjugation of X and Z may be carried out by using chemical ligation.
Amide-forming chemical ligation at positions other than cysteine has been reported.
Non-enzymatic ligation may involve a chemical ligation.
The original chemical ligation methods involved the formation of a non-native bond at the ligation site.
Figure 1 illustrates schematically the general principle of chemical ligation.
The principle of chemical ligation is shown schematically in Figure 1.
Alternatively additions may be made by fragment condensation or native chemical ligation e . g.
As disclosed herein, native chemical ligation is limited to reaction at an N-terminal Cys residue.
Mathieu has extensive experience in peptide synthesis, chemical ligation and bioconjugation.
The native chemical ligation procedure is limited to reaction at an amino-terminal Cys residue.
In a preferred method of preparation, the hydrogel is generated through chemical ligation reactions.
Chemical ligation methods are well known in the art, e . g.
Conjugates of hydroxyalkyl starch and an active substance, prepared by chemical ligation vía thiazolidine.
FIG . 9 represents a chemical ligation reaction of the thiazolidine type using a peptide according to the invention ;.
This enables the use of large recombinant protein-derived thioesters in native chemical ligation.
The method of claim 1, wherein said chemical ligation step comprises,.
In this procedure, two polypeptides are first synthesized, and contain termini adapted for chemical ligation.
Chemical ligation dependent probe amplification ( CLPA ).
An EU project prioritised development of new, environmentally friendly and versatile chemical ligation methods.
Methods for chemical ligation are disclosed, for example, in U. S.
Recombinant expression can also be used to give a Cys-polypeptide for use in native chemical ligation.
Figures 22A -22C provide exemplary schematics of chemical ligation with orthogonal chemistry.
Native Chemical Ligation ( NCL ) of fragment peptides,.
Proteins of up to 300 amino acids are produced by a convergent approach using chemical ligation.
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Tubal ligation was performed as previously informed consented
Another method involves ligation vía cohesive ends
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Examples of using Chemical
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Speciation of chemical species should be considered in inventories
They hit me with some kind of chemical
Status of the chemical under international conventions
