Examples of 'chloride gradient' in a sentence
Meaning of "chloride gradient"
chloride gradient: This scientific jargon refers to the difference in chloride concentration between two points, often in a laboratory or biological context. It is commonly used to describe the variation in chloride levels across a gradient, which can have various effects on processes
How to use "chloride gradient" in a sentence
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chloride gradient
Elution is done using a sodium chloride gradient as described above.
A sodium chloride gradient was again employed to elute the proteins.
Ultracentrifugation on cesium chloride gradient has many drawbacks.
A sodium chloride gradient was used to elute various modified hemoglobins.
Virus stocks are then prepared on a cesium chloride gradient.
A cesium chloride gradient was then performed.
The final purification was performed in a cesium chloride gradient.
Epo purified on a caesium chloride gradient as described in Example 2 ( 1 single cycle of infection ).
The plasmid DNAs are purified on a cesium chloride gradient.
Plasmid DNA was prepared by alkaline lysis and cesium chloride gradient centrifugation by standard methods.
The DNA was precipitated in ethanol and purified by isopycnic banding on a cesium chloride gradient.
The plasmid DNA is isolated on a cesium chloride gradient as described above.
The DNA was precipitated with ethanol and purified by isopycnic banding on a cesium chloride gradient.
A suitable concentration range for the sodium chloride gradient may be from about 0.0 M to about 1 M NaCl.
The mixture was then centrifuged through a cesium chloride gradient.
See also
Sample is eluted using an increasing, step-wise sodium chloride gradient in the pH 7.4 Tris / PMSF buffer.
Both xyloglucanase and CMCase bound to the column and was eluted using a 1N sodium chloride gradient.
Another method used to separate DNA is cesium chloride gradient ultracentrifugation.
Purification was achieved by preparative cation-exchange chromatography, eluting with a linear 0-500 mM sodium chloride gradient.
The column was then eluted with a linear 0 - 0.3 M sodium chloride gradient in the same Tris / urea buffer.
A small peak is observed upon elution with the magnesium chloride gradient.
The proteins were then eluted using a linear sodium chloride gradient ( 0 -1 M Nacl ) in Buffer A.
A prominent peak is observed upon elution with the magnesium chloride gradient.
The viral particles are then purified by double cesium chloride gradient purification.
Evaporate the solvent and purify on a silica gel column eluting with a 0-10 % methanol / methylene chloride gradient.
After filtration, the deprotected oligonucleotide was purified by sodium chloride gradient and ion-exchange chromatography.
The clones were then amplified and the DNA was purified on a cesium chloride gradient.
The DNA was purified on a cesium chloride gradient.
The anion exchange column was eluted with an increasing sodium chloride gradient.
Viruses were propagated in 293 cells and purified by two rounds of cesium chloride gradient ultracentrifugation.
The endoglucanase will bind and can be eluted using a sodium chloride gradient.
Plasmids used for transfection were purified from bacterial cultures by caesium chloride gradient centrifugation.
The recombinant adenovirus was isolated by plaque purification followed by double cesium chloride gradient centrifugation.
For further experimentation the viruses were prepared on a larger scale by cesium chloride gradient centrifugation.
Modified Hbs were eluted with a sodium chloride gradient.
Total RNA was subsequently purified on a caesium chloride gradient.
Plasmid DNA was purified by centrifugation through a cesium chloride gradient.
The DNA was purified by isopycnic banding on a cesium chloride gradient.
The PEG mutein is then eluted from the column with a sodium chloride gradient.
During my thesis I showed a delay in the development of the chloride gradient.
In both cases, phytase was eluted in pure form by way of a sodium chloride gradient.
More concretely, the methods used are the alkaline lysis method and caesium chloride gradient purification.
After loading the protein ( s ) on the column, bound materials were eluted by a sodium chloride gradient.
The calf thymus histones were also subjected to chromatography in a sodium chloride gradient ( 0 to 2 M ).
It is washed with equilibration buffer and then IL-21 is eluted with a sodium chloride gradient.
The xyloglucanase bound to the column and was eluted using a 0.5 M sodium chloride gradient.
The method of claim 1, wherein the elution conditions comprise utilizing a linear 5 - 0M sodium chloride gradient.
CBPs are eluted with either with 0.5 M choline chloride or with a linear choline chloride gradient.
When anion exchange chromatography is performed, a subsequent 0-500 mM sodium chloride gradient is employed.
Alternatively, sodium acetate buffer, pH 4.5, in conjunction with a sodium chloride gradient can be employed.
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