Examples of 'chondroitinase' in a sentence
Meaning of "chondroitinase"
chondroitinase (noun): Chondroitinase is an enzyme that helps break down chondroitin sulfate, a component of cartilage in the body
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- Any of a class of enzymes that catalyse the hydrolysis of chondroitin sulfate proteoglycans.
How to use "chondroitinase" in a sentence
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chondroitinase
In a further embodiment the chondroitinase is a recombinant chondroitinase.
The chondroitinase may be further processed by drying or lyophilization.
Interpositional nerve grafting for chondroitinase experiments.
Chondroitinase may be administered locally or systemically.
Methods of purifying chondroitinase are also described.
Preparation of acellular nerve grafts treated with chondroitinase.
In another embodiment storage of chondroitinase is in the lyophilized form.
Divalent metal salts may inhibit an activity of chondroitinase.
Chondroitinase activity can be stabilized by the addition of excipients or by lyophilization.
This bond disappears upon digestion with chondroitinase.
The present example illustrates exemplary chondroitinase mutant enzymes of the present invention.
Compositions useful in the method include acceptable formulations of chondroitinase.
Cells that express catalytically active chondroitinase are cloned and expanded.
Storage buffer pH does not affect the activity of the purified chondroitinase.
Release was monitored by measuring chondroitinase activity released from the matrices over time.
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Chondroitinase as described above has been conventionally directly injected into the intervertebral disc.
Remove the lid of the jar and decant the chondroitinase solution.
Chondroitinase is now one of the hottest regenerative therapies for brain and spinal cord.
No protease activity is detected in such a chondroitinase ABC.
The chondroitinase may be captured from the cell extract using ion exchange chromatography.
In a further embodiment the chondroitinase is chondroitinase AC.
Chondroitinase ABC has been shown to degrade glial scars.
The column was washed and the chondroitinase AC was eluted.
Purified chondroitinase ABC having such properties are particularly preferred.
Determination of isoelectric point for the recombinant chondroitinase ABCI.
Chondroitinase ABCI enzymatic activity was assayed as described elsewhere herein.
Gel filtration was used as a polishing step for chondroitinase ABCI.
Chondroitinase ABC activity is confirmed by digesting a sample of purified chondroitin sulfate.
The fractions that revealed a relatively pure chondroitinase AC were pooled.
In vitro use of chondroitinase ABC for promoting elastic fiber formation.
It is possible to use known methods for formulation of the chondroitinase ABC.
A chondroitinase ABCI was used as an example of a chondroitinase that can be purified and formulated.
Bovine serum albumin treated with chondroitinase ABC did not shown any inhibition.
Chondroitinase ABC can be provided in a dosage form that is usually employed as an injection.
Compositions and methods of using chondroitinase ABCI mutants.
The activity of the chondroitinase ABCI enzyme was a relatively high enzyme preparation.
Analytical SEC was used to characterize aggregation and conformation of the chondroitinase ABCI.
The chondroitinase ABCI was eluted with the expected retention times without substantial loss in sample.
The elution profiles of recombinant chondroitinase ABCI and the native chondroitinase ABCI were the same.
Chondroitinase may be administered topically, locally or systemically.
Optionally, a dialysis step to further purify the chondroitinase is used.
One nerve was injected with chondroitinase ABC and the contralateral nerve received vehicle alone.
In another aspect of the invention, the formulations consistently provide stable chondroitinase activity.
The recombinant chondroitinase ABCI of had a retention time and molecular weight expected for a chondroitinase ABCI.
In one embodiment, the enzymatic compound is a chondroitinase enzyme produced by bacteria.
Treatments with chondroitinase AC II or hyaluronidase only partially reduced the binding.
The unmodified hyaluronan-degrading enzyme can be a chondroitinase or can be a hyaluronidase.
A recombinant chondroitinase encoded by the nucleotide sequence of Sequence ID No.
By contrast, the present inventors report the surprising finding that chondroitinase also promotes neuronal plasticity.
Particularly, chondroitinase is more preferably used.
