Examples of 'clarified lysate' in a sentence
Meaning of "clarified lysate"
clarified lysate - Refers to the process of making a clear or more understandable version of a lysate, which is a liquid containing the products of cell lysis, such as proteins and nucleic acids
How to use "clarified lysate" in a sentence
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clarified lysate
Set aside any remaining clarified lysate.
The clarified lysate is then filtered at least once.
The supernatant was called the clarified lysate.
The clarified lysate contains the plasmid DNAs.
The filtrate is the clarified lysate.
The clarified lysate is then concentrated and fractionated by chromatography.
A fifth tank is used for a holding the clarified lysate or clarified plasmid containing fluid.
The clarified lysate was collected for further processing and the pellets were discarded.
Preferably, the cellular component of interest is purified from the clarified lysate.
Assay clarified lysate.
Preferably, the cellular component of interest is a plasmid that is present in the clarified lysate.
The clarified lysate may be then concentrated by ultrafiltration e . g.
Polysaccharide was purified from the clarified lysate using standard procedures ( as described above ).
The clarified lysate was subjected to size-exclusion chromatography.
Once the centrifugation pellet is removed and the supernatant recovered, it constitutes the clarified lysate.
See also
Instead, the clarified lysate had been treated with high levels of nuclease.
The expected virus concentration factor at this point is 18X from clarified lysate.
The clarified lysate was loaded onto an anti-intrinsic factor antibody affinity column.
The turbidity of the clarified lysate was less than 2 NTU.
The clarified lysate was concentrated to about 1/8th original volume.
After centrifugation and filtration, the clarified lysate contains the plasmid DNA.
The clarified lysate was then concentrated by ultrafiltration with a 300 kDa cutoff membrane.
Lanes 4 and 5 are samples of clarified lysate.
Preferably, the clarified lysate is purified by size-exclusion chromatography.
Lane 4 is a sample of clarified lysate.
Cpn10 was purified from the clarified lysate by ion-exchange and hydrophobic interaction chromatography.
The retentate was diafilteced with 3 volumes of Diafiltration Buffer ( below ) to produce the clarified lysate.
In the same manner, 3 ml of clarified lysate was loaded onto a Ni-NTA gravity flow column.
The retentate was diafiltered with 3 volumes of Diafiltration Buffer ( below ) to produce the clarified lysate.
Lanes 2 and 3 are clarified lysate before and after incubation with amylose resin, respectively.
Crude lysate was recovered and filtered as before, yielding 85 L of clarified lysate.
Alternately, clarified lysate may be retrieved from tank 401 and collected in an intermediate vessel.
Celpure ™ is then mixed into the clarified lysate at roughly 10 g / L.
Preferably, clarified lysate is retrieved from tank 401 and subjected to microparticulate filtration as a continuous operation.
GST-CERT fusions are purified from clarified lysate with glutathione resin ( GE Healthcare ).
The clarified lysate was concentrated to 75ml and the concentration permeate collected in a 2L bottle.
Using centrifugation to remove cell debris Clarified lysate was produced by alkaline lysis in a 100g preparation.
The clarified lysate is adjusted to a pH of 5.3 and a conductivity of 3 mS / cm.
Approximately 106 L of clarified lysate was recovered.
The clarified lysate was then centrifuged overnight at 83,000 x g and the VLP band was collected.
Fill reservoir with approx 1.8L clarified lysate and connect butt containing lysate to system.
The clarified lysate from Example 2 was stored at 4 °C for 6 days.
Three milliliters of clarified lysate was loaded onto a Co2 + CM-Asp chelating resin gravity flow column.
The resulting clarified lysate was then ready for down-stream processing . Down-Stream Processing.
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Examples of using Clarified
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Flushing system with clarified aerated slurry
She clarified that there was no ambush here
This issue should be clarified in the commentary
Examples of using Lysate
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The clear lysate was deposited on the column
A preferred sample according to the invention is a lysate
Production of a crude lysate possessing enzymatic activity
