Examples of 'cleavage agent' in a sentence
Meaning of "cleavage agent"
cleavage agent: a chemical substance or component used in scientific experiments or industrial processes to induce cleavage or separation of materials
How to use "cleavage agent" in a sentence
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cleavage agent
The cleavage agent may be an enzyme.
Cleaving the captured proteins with a sequence specific cleavage agent.
Preferred cleavage agents include an endopeptidase or a chemical cleavage agent.
A cleavable peptide linker can be cleaved by an enzymatic or a chemical cleavage agent.
The cleavage agent added is dependent on the cleavage group present.
The captured proteins may then be cleaved with a sequence specific cleavage agent.
The cleavage agent acts independently of sequence of the cleavage structure.
Incubating the cleavage structure and cleavage agent under conditions wherein cleavage can occur.
The cleavage agent may be a chemical cleavage agent such as cyanogen bromide.
Washing away peptides generated by the cleavage agent not retained on the solid phase support.
The cleavage agent can be any agent that is capable of cleaving invasive cleavage structures.
Optionally said vector comprising said transgene further comprises a nucleic acid sequence encoding the cleavage agent.
A different sequence specific cleavage agent being used in the generation of each individual profile.
In certain embodiments, a protease is a chemical cleavage agent.
It is a good cleavage agent for footprinting because its size makes it easily physically hindered.
See also
In related embodiments, the agent comprises a cleavage agent.
The cleavage agent must recognise a specific amino acid residue or sequence of amino acids reliably.
Preferably, the cleavage agent is DNAPTaq.
In some embodiments, the agent comprises a cleavage agent.
The cleavage structure and the cleavage agent are incubated under conditions wherein cleavage can occur.
Preferably, said composition comprises the cleavage agent.
Contacting the sample with a cleavage agent to generate polypeptide fragments ;.
After the annealing reaction, the annealed molecule is exposed to a cleavage agent.
In related embodiments, the cleavage agent comprises a structure-specific nuclease.
Preferably, the population of polypeptides is immobilised before treatment with the cleavage agent.
In some preferred embodiments, the cleavage agent comprises a structure-specific nuclease.
Contacting the C-terminally modified proteins with a sequence specific cleavage agent.
In a preferred embodiment, the cleavage agent recognition site is a thrombin recognition site.
A method according to any one of the preceding claims, wherein the cleavage agent comprises a peptidase.
A suitable cleavage agent will be able to cleave a bifurcated duplex in a sequence-independent manner.
Contacting a sample comprising polypeptides with a first cleavage agent to generate polypeptide fragments ;.
The cleavage agent may be natural, synthetic, unmodified or modified.
In this disclosure ( not according to the invention ), the cleavage agent may be trypsin.
Preferably, the cleavage agent is a peptidase, such as a serine protease, preferably trypsin.
A preferable example of a cleavage agent is a 5 ' nuclease activity of DNAP, such as DNAPTaq.
In such embodiments, a cleavage agent is referred to herein as a " cleavage-inducing moiety . ".
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