Examples of 'cloning steps' in a sentence
Meaning of "cloning steps"
cloning steps - the process of creating genetically identical copies of an organism or a DNA sequence through scientific means
How to use "cloning steps" in a sentence
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cloning steps
This site was used in subsequent cloning steps.
No cloning steps are required prior to sequencing.
These sites were used in the subsequent cloning steps.
In particular no cloning steps are required.
The alternative splicing cassette is created through a series of cloning steps.
In further cloning steps this transcription initiation region was replaced by that of gsiB.
Prokaryotes are the preferred host cells for initial cloning steps.
The different cloning steps are represented by the alignment of the different cDNA fragments obtained.
The first construct was made using three sequential cloning steps.
This approach can circumvent multiple cloning steps where multiple domains are selected for mutagenesis.
A wide variety of cloning vectors is available to perform the necessary cloning steps.
E involved several cloning steps that produced 4 intermediate constructs pGOSA.
Prokaryotes may be typically used as host cells for initial cloning steps.
Example 3 illustrates the cloning steps to provide an expression vector encoding the trypsin variant.
A wide variety of cloning vectors are available to perform the necessary cloning steps.
See also
The various cloning steps are indicated in Figure 5.
The resulting vector was used as target vector for the following cloning steps.
The various cloning steps leading to the construction of these plasmids are presented in FIG . 4.
Prokaryotes are the preferred host cells for the initial cloning steps of this invention.
The various cloning steps leading to the construction of these plasmids are presented in Figure 4.
The Eco RI enzyme recognition site was added to facilitate subsequent cloning steps.
Cloning steps are carried out on noninducing M0 medium.
Prokaryotic Cells Prokaryotes are the preferred host cells for initial cloning steps.
Selection and cloning steps were performed on methylcellulose-based semi-solid in 96 well plates.
Cloning of a gene of interest is achieved in only 2 cloning steps.
The various cloning steps are indicated in FIG . 5.
Escherichia coli DH5a was used for some cloning steps.
Two cloning steps were necessary to derive these vectors containing 1.3 unit of HBV genome.
In some embodiments, the sequencing method includes one or more cloning steps.
The different amplification and cloning steps are shown in FIG . 2.
The products of a minimum of 5 third round reactions were pooled for the cloning steps.
A further three cloning steps lead to the final expression plasmid pAR8 ( Figure 14 ).
The cloning sites derived from pUC19 were later on used for further cloning steps.
Figure 6 shows the various cloning steps.
Most preferably, the methods of the present invention do not require any cloning steps.
Alternatively, the non-natural mutations may be introduced after the cloning steps of iii and / or iv.
The genetic stability of the pre-frozen-material was monitored during the various cloning steps.
In order to generate the vector cPPT-C ( FVIIIAB ) IGWS, three cloning steps were required.
A viral vector containing a green fluorescence protein ( GFP ) gene was made in several cloning steps.
FIG . 6 collates the various cloning steps.
The primers have EcoR1 and Pst1 sites respectively on the ends for further cloning steps.
A viral vector containing the T7 RNA polymerase coding sequence is made in several cloning steps.
Head-to-tail orientations generally require multiple cloning steps.
Primer P063 contains the sequence for an additional Mlul restriction site used in later cloning steps.
Three other constructions ( ~ 22 kbp ) were derived from the intermediate cloning steps.
Was first constructed from vectors pSI ( Promega ) and pEF / Bsd ( Invitrogen ) through multiple cloning steps.
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Examples of using Cloning
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The cloning of human beings shall be prohibited
It is essential that reproductive cloning be categorically prohibited
Human cloning raises highly sensitive issues
