Examples of 'clontech' in a sentence
Meaning of "clontech"
clontech (noun) - a company specializing in the development and manufacturing of research reagents and tools for molecular biology studies
How to use "clontech" in a sentence
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clontech
Green fluorescent protein was purchased from Clontech.
Amplification products were purified using a Clontech DNA amplification cleanup kit.
The actin cDNA fragment was purchased from Clontech.
The Clontech reverse transcriptase and dNTPs were substituted with Superscript II and dNTPs from Invitrogen.
A human cDNA library was bought from Clontech.
BacPAK expression system ; Clontech for expression in insect cell lines such as SF9 cells.
Human heart cDNA library was purchased from Clontech.
Hybridization of Clontech cDNA Arrays with dUTP-labeled cDNA Probes was carried out as follows,.
The filters were washed according to the Clontech instruction.
PCR was performed using Clontech Advantage 2 Polymerase mix following the manufacturer 's recommended protocol.
The human multiple tissue mRNA blot was purchased from Clontech.
Human small-intestine total RNA obtained from Clontech was also reverse-transcribed in the same fashion.
The blots were hybridized with the ExpressHyb hybridization solution Clontech.
Reagents are available from Clontech in their PCR-direct Cloning System.
Total or mRNA from different human tissues are commercially available from Clontech.
See also
The RetroExpress ® system, available from Clontech is especially useful.
Vectors for the Tet On gene expression system were obtained from Clontech.
The Clontech kit is designed to carry out PCRs on GC-base-rich genomes.
The human multiple tissue blot was obtained from Clontech and probed as specified.
First strand cDNAs from 16 different normal human tissues were obtained from Clontech.
Foxp3 cDNA is cloned into an eGFP Clontech plasmid to create a Foxp3-GFP fusión protein.
This vector was purchased from Clontech.
Clontech ( san Diego CA ) supplied the amino modifiers.
Human cDNAs from tissues were purchased from Clontech or Invitrogen.
Clontech CDS primers specific to the 268 genes on the Atlas â„¢ human cytokine / receptor array Cat.
Actin probes were supplied by Clontech.
Clontech ( San Diego, California ) supplied the amino modifiers.
Membranes were purchased from Clontech.
Single-stranded human liver cDNA can be obtained from Clontech PCR-ready Quick Clone cDNA, Cat.
Cell culture reagents were obtained from Fisher Scientific and molecular biology reagents from Clontech Inc.
The methodology used is that recommended by Clontech ( Palo Alto, USA ), and comprises,.
Multiple tissue Northern filters were obtained from Clontech.
Hybridization was for one hour at 68°C using Clontech " ExpressHyb Hybridization Solution ".
Commercially available cDNA libraries such as cDNA library from Clontech Lab.
Coli DH5a competent cells were purchased from Clontech Laboratories ( Palo Alto, CA ).
First strand cDNAs from 16 different normal human tissues can be obtained from Clontech.
In the vectors, inserts corresponding to GFP sequence taken from Clontech vector pEGFP-N1 are used.
Body map profiling also is carried out, using total RNA panels purchased from Clontech.
A human intestinal cDNA library was obtained from Clontech ( Palo Alto, CA ).
Aliquots of the supernatants were assayed for B-galactosidase activity as directed by Clontech.
For example, genomic and cDNA libraries can be purchased from Clontech Laboratories Inc.
A commercially available bacteriophage lambda gt10/bovine small intestine cDNA library was purchased from Clontech.
Human genomic libraries were obtained from Clontech ( Palo Alto, USA ).
The filter assay for B-galactosidase was done as recommended by Clontech.
IRES was cut out from pIRES purchased from Clontech ( Palo Alto, CA ).
In addition, a human multiple tissue Northern blot was purchased from Clontech.
GFP expression vector, pLEIN was purchased from Clontech ( Palo Alto, CA ).
CDNA was prepared from human testis polyA+ RNA that was obtained from Clontech.
Cerebral cortex total RNA was purchased from Clontech ref.
Useful plant binaries vectors include BIN19 and its derivatives available from Clontech.