Examples of 'co-transfection' in a sentence
Meaning of "co-transfection"
Co-transfection is a scientific technique involving the introduction of multiple DNA molecules into a cell simultaneously
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- Alternative spelling of cotransfection
How to use "co-transfection" in a sentence
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co-transfection
Co-transfection efficacy is determined using reference compounds.
This is preferably accomplished by co-transfection of a plasmid encoding the recombinase.
Co-transfection of additional expression constructs is indicated in each example.
Functional activity was assessed using a luciferase co-transfection assay.
Co-transfection of cells may be accomplished using standard techniques.
Morphology of cells was visualized with co-transfection with mCherry construct.
The co-transfection with an alternative spliceotype does not complement the loss.
Recombinant virus was generated by co-transfection with a plasmid containing a complete AAV-genome.
Co-transfection of heavy and light chain expression vectors in appropriate cell lines.
The selection system typically employs co-transfection of a polynucleotide sequence encoding a selection marker.
The co-transfection assay can detect small molecule agonists or antagonists of target IRs.
The selectable marker gene can be introduced into the same cell by co-transfection.
In this regard, the co-transfection assay mimics an in vivo system in the laboratory.
The selectable marker can optionally be present on a separate plasmid and introduced by co-transfection.
Such co-transfection strategies likewise enable selection as is well-known in the prior art.
See also
Clones from cells transfected only with pGK-neo were isolated as controls for co-transfection.
Expression of the first co-transfection plasmid results in production of the intracellular receptor protein.
The heavy and light chains were cloned into individual expression vectors to allow co-transfection.
This allows co-transfection of two vectors expressing different proteins into one cell.
Antibodies were expressed transiently by co-transfection into CHO cells.
The co-transfection assay is therefore useful to detect both agonists and antagonists of specific IRs.
A third alternative utilises co-transfection of unlinked DNA fragments.
Co-transfection major peaks eluting from the column were collected for analysis by mass spectrometry.
In vitro studies utilizing the co-transfection assay have also shown this same synergistic effect.
Co-transfection data showing repression of luciferase reporter activity vÃa VEGF-KRAB protein expression.
Testing GFP expression regulation in transient co-transfection with TRAP-expression plasmids.
These co-transfection data demonstrate that ZFPs can be used to regulate expression of reporter genes.
The number of blue cells can be partially restored by co-transfection of Bak with Bcl-xL.
The co-transfection results in the recombination of the both plasmid DNAs into the viral genome.
Hence, the tandem constructs may be as effective as co-transfection.
This co-transfection assay is a surrogate assay for in vivo calcemic activity of VDR ligands.
The NL-RLuc virus was prepared by co-transfection of two plasmids, pNLRLuc and pVSVenv.
This co-transfection results in the recombination of the plasmid DNA into the viral genome.
No staining cells were observed when pCIneoL ( c ) was replaced by pCIneo in the co-transfection experiments.
The effects of Rep co-transfection on the integration of circular intermediate plasmids is also evaluated.
The rtPA expression plasmid was included in the co-transfection mixture at a 10fold molar excess.
Transient DNA co-transfection is being used to test the intracellular effectiveness of the chimerical ribozyme.
A recombinant baculovirus was obtained after co-transfection of the pIP596 construct with wild type virus.
After co-transfection into recipient CHO cells, the resulting colonies may be selected using both markers.
Anti-tumor activity was determined by measurement of inhibition of luciferase activity following co-transfection with LucSV40.
First is described the co-transfection of porcine and human factor VIII sequences on separate vectors.
Cells were subjected to FACS analysis 24 hours after co-transfection.
The effect of co-transfection of the MAR has not found to be saturated See Fig.
Thus, we investigated superoxide generation by NOX3 upon co-transfection with cytoplasmic subunits.
This simple co-transfection method enabled the evaluation of DNA synthesis in cells expressing exogenous DNA.
An expression clone of ferritin-H represses this B-globin promoter in transient co-transfection experiments.
Lentiviruses were packaged by co-transfection of constructs with the Sigma-Aldrich MISSION Lentiviral Packaging Mix.
Ferritin-H represses expression driven by the b-globin promoter in transient co-transfection assays.
FXR Co-Transfection Assay The basic co-transfection protocol for measuring FXR activity is as follows.
In yet another example, beta-galactosidase co-transfection assays can be used for determination of cell death.
