Examples of 'coce' in a sentence
Meaning of "coce"
Coce (noun): A term that does not appear to have a widely recognized definition in English. It may be a specific name or reference that is not commonly used
How to use "coce" in a sentence
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coce
Some were able to do this in the absence of CocE.
The expressed mutant CocE polypeptide is isolated or displayed.
This enzyme is markedly less effective than CocE.
The mutant CocE polypeptide is expressed in the cell.
Circular dichroism of wild type and mutant CocE.
Endotoxin contamination of CocE mutants can be performed in several ways.
Compounds that thermostabilize CocE.
In some embodiments the CocE polypeptide is in a pharmaceutically acceptable carrier.
Further described herein are methods of producing the mutant CocE polypeptides.
Mutant CocE polypeptides can be encapsulated and administered in a variety of carrier delivery systems.
Directed evolution can also be used to generate thermostable CocE mutants.
Nucleic acid molecules encoding mutant CocE fusion proteins are also within the invention.
There is a need for additional methods and compositions for thermostabilization of CocE.
Mutagenesis of the CocE host organism can also be used to generate thermostable CocE mutants.
Embodiments of the invention further pertain to methods of producing the mutant CocE polypeptides.
See also
Protection from aggregation of thermal stable CocE mutants was assessed using size exclusion chromatography.
Another aspect of the invention disclosed herein concerns recombinant forms of the CocE polypeptide.
The gene for CocE has been isolated and cloned Bresler et al.
Several high throughput screening methods for identification of thermostable CocE variants were implemented.
A direct ELISA specific for CocE antibodies was set up by a standard protocol.
RBC encapsulation can also be carried out by using pegylated mutant CocE.
Such expression procedures can be utilized for the mutant CocE polypeptides described herein.
Pegylation of mutant CocE can also be used in conjunction with RBC encapsulation.
CLEANING uf flushing uf rinsing uf washing coce coes USE soft drinks.
WT CocE in the presence of excess benzoic acid FIG.
Thermally stable mutant CocE polypeptides Mutant.
CocE is stable when being stored in DTT Turner et al.
RBC encapsulation of CocE can be accomplished via a linked PTD peptide.
Figure 2 is a ribbon diagram illustrating the CocE structure.
Identification of CocE thermostable mutants by structure-based analysis.
Analysis of thermostability of various compounds with wild-type and a mutant CocE.
In various embodiments, the mutant CocE polypeptides are pegylated.
Wild-type CocE showed superior catalytic efficiency over human BChE.
In one embodiment, small molecules are used to thermostabilize the mutant CocE polypeptides disclosed herein.
Also, random mutant CocE polypeptides can be screened for the desired characteristics.
Such results point to the importance of extending the thermostability of the wild-type CocE.
In various embodiments, the mutant CocE polypeptide is encapsulated in a red blood cell.
As such, RBC encapsulation can be utilized to shield thermostable forms of CocE from clearance.
Although CocE is a dimer in solution and in crystals, the modeling was performed with a monomer.
The resulting increase in thermostability of the mutant CocE polypeptide is at least about 2 kcal / mol.
The mutant CocE polypeptide of claim 1, wherein the mutant CocE polypeptide is pegylated.
Phenylboronic acid increased the half-life of the CocE the most, followed by benzoic acid.
Generally, mutant CocE polypeptides can be initially designed according to the approaches described above.
Thus, PTD peptide conjugation to mutant CocE can facilitate RBC encapsulation.
The mutant CocE polypeptide of claim 1, wherein at least two amino acid residues are substituted.
Thermo-instability contributes to the short plasma half-life of wild-type CocE.
In some embodiments, the thermostable mutant CocE polypeptides have less esterase activity than wild-type CocE.
FIG . 8 is a graph showing benzoic acid thermostabilization of CocE.
Rational design of mutant CocE described herein was based upon molecular dynamics ( MD ) simulations.
FIG . 2 is a ribbon diagram illustrating the CocE structure.
