Examples of 'colony hybridization' in a sentence
Meaning of "colony hybridization"
Colony hybridization: A molecular biology technique used to identify colonies of bacteria or other microorganisms that contain specific nucleic acid sequences. This technique involves transferring DNA or RNA to a membrane and using a labeled probe to detect the target sequences
How to use "colony hybridization" in a sentence
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colony hybridization
Transformants are screened by colony hybridization on nitrocellulose filters.
Selection of cephalosporin acetylhydrolasepositive clone by colony hybridization.
Colony hybridization was performed using the transformant as a library.
Tetracycline resistant colonies were screened by colony hybridization.
Colony hybridization is a routine procedure in the art of molecular biology.
Pure clonal isolates were subsequently identified by colony hybridization.
Colony hybridization or plaque hybridization was conducted using the transformant as a library.
Single hybridizing clones were isolated by standard colony hybridization techniques.
The probes used in the colony hybridization analysis are set forth below.
Colony hybridization was carried out using the transformant thus obtained as a library.
All the obtained transformants were purified and analyzed with colony hybridization.
Filters were processed for colony hybridization according to the method of Maniatis et al.
The following oligonucleotides were synthesized as probes for use in the colony hybridization studies.
The colony hybridization was conducted by a known procedure described in a literature J.
These authors reported methods for effective colony hybridization directly on HGMFs.
See also
Colony hybridization was performed using a random-primed probe corresponding to the HIS3 gene.
The labelled fragment was used to probe the filters by standard colony hybridization techniques.
As the isolation method, the colony hybridization method described previously may be used.
Three isolated clones were identified and streaked out for single colony hybridization.
The Lys+ clones were screened by colony hybridization for the presence of mu.
Its presence in the genome of Streptomyces can only be detected by colony hybridization.
FIGURE 6 is a picture of an autoradiogram depicting colony hybridization of putative clones of dTMPase-encoding DNA.
Secondary screening was performed for thus obtained hybridization-positive colonies by colony hybridization.
Thus, both gel electrophoresis and colony hybridization are eliminated.
Extended cDNAs containing the 5 ' EST sequence are identified by colony PCR or colony hybridization.
Strong cross hybridization in in situ colony hybridization with the Bam HI insert of pAGC1.
Transformants were screened for insert-containing clones by colony hybridization J.
This radioactive probe was used for colony hybridization to the gene bank of S. lydicus.
Colony hybridization techniques are disclosed in Sambrook et al ., supra.
The resulting PCR products were then used as probes in colony hybridization screening.
Colony hybridization is carried out as described by Grunstein et al ., Proc.
Colonies containing the Nsi I insert were identified by colony hybridization and autoradiography.
Colony hybridization is performed as described by the same authors ( page 312 ).
YAC clones containing these RFLP markers were identified by colony hybridization experiments.
The detailed procedure for colony hybridization is described in reference 9.3.
EXAMPLE 2, Same as example 1 except that testing of the strains is by colony hybridization.
All colonies were screened by colony hybridization to the A1 probe based on amino acids 1-6.
Ampicillin-resistant and tetracycline-sensitive transformants are screened by colony hybridization using the PL20 probe.
Colony hybridization is performed as described by the same author ( page 312 ).
Virotyping of the coliPROtec strain was done by colony hybridization and / or polymerase chain reaction ( PCR ).
Colony hybridization was carried out under the condition of 36°C for 40 hours.
Colonies containing inserted blocks are identified by colony hybridization with ³ ² P-labelled oligonucleotide probes.
Nylon membrane Hybond-N + ( Amersham, England ) was used for DNA transfer for colony hybridization.
Approximately 4,000 clones were screened vía colony hybridization using the aforementioned DNA fragments as probes.
Identification of L. salivarius PS2 in the milk samples was done by a DNA-DNA colony hybridization assay.
All colonies were screened by colony hybridization to the Al probe ( FIG . 1 ).
Verification of Plasmid pHDM174-Transformation and colony hybridization.
All colonies were screened by colony hybridization to the A1 probe ( Figure 1 ).
Clones carrying YHV303-A6 derived inserts were identified by a two-step colony hybridization procedure.
These include Southern Blots, Northern Blots, colony hybridization procedures, and dot blots.
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Examples of using Hybridization
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There was no evidence of hybridization in the wild population
The hybridization is carried out in closed loop
Method and machine for hybridization by refusion
