Examples of 'column buffer' in a sentence
Meaning of "column buffer"
column buffer - In programming, a column buffer is a reserved memory space used to store temporary data while processing columns of text or data
How to use "column buffer" in a sentence
Basic
Advanced
column buffer
Unbound proteins were removed by washing with column buffer.
The column buffer was allowed to drain from the column by gravity.
Cells were pelleted and lysed by sonication in column buffer.
The column is equilibrated in column buffer and run at an appropriate flow rate.
Resuspend pellet in 1 ml cold column buffer.
Additionally, the SE-HPLC column buffer was adjusted to the same pH for analysis.
Further, the pH and ionic strength should be similar or identical with the column buffer.
The DNA pellets are resuspended in column buffer prior to gel filtration chromatography.
The recombinant protein was eluted by a gradient of 0 to 1 M NaCl in column buffer.
The columns were washed with 10 mls each of Column Buffer and the washes were collected.
Briefly, lectin gels were equilibrated with twice 5 mL of column buffer.
The GUS activity is eluted with column buffer containing 200 mM methyl mannopyranoside.
The resin is washed with 60 ml of column buffer.
The column buffer was obtained by diluting the 2.5M carbonyldihydrazide solution to 2M with water.
Columns were washed with 10 ml of column buffer.
See also
Wash the column with column buffer and monitor the OD at 280 nm.
Unbound recTSH was collected by 10 repeated centrifugations with 1 mL column buffer.
The sterile filters were rinsed with fresh column buffer to a target bulk weight of 14.6 Kg.
The column was then washed with 20 volumes of column buffer.
Wash with 3 column volumes column buffer without Tween 20.
Following sample addition the column was washed with 20 mL of column buffer.
Column equilibrated in column buffer containing 1.5 M NaCl.
The sample was loaded on the column and eluted with 3.5mL of the column buffer.
The column was eluted with column buffer containing 0.5 M N-acetylglucosamine.
The supernatant was loaded onto a Ni-NTA affinity column ( Qiagen ) pre-equilibrated with column buffer.
The bound MBP-L chain fusión protein was subsequently eluted with column buffer containing 10 mM maltose.
The column was washed five times with 0.5 ml of column buffer.
The column was washed five times with 0.5mI of column buffer.
You'll also be interested in:
Examples of using Buffer
Show more
No buffer zones are defined for the site
The boundary of the buffer zone is adequate
The buffer has had too much
Examples of using Column
Show more
This column also captures those situations
This marks my final column in this magazine
A column matrix is a matrix with a single column
