Examples of 'column purification' in a sentence
Meaning of "column purification"
column purification: In scientific or chemical contexts, 'column purification' refers to a process of separating and purifying substances using a column filled with a stationary phase material. It is a common technique used in chromatography to isolate compounds based on their properties
How to use "column purification" in a sentence
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column purification
Each column purification is run at room temperature.
The final product was purified by column purification.
Column purification of crude material afforded pure product as oil.
The synthesis foresees a column purification of the final product.
Spin column purification can be used to remove free nucleotides.
The shortened chains are later easily removed in column purification.
Finally a last column purification gives a converted target ready to be amplified.
Rabbit antiserum was cleared of lipid prior to column purification of polyclonal IgG.
Column purification gives a yellow-colored oil.
This step was repeated two more times and was followed by a column purification.
In the third approach the column purification can be combined with ExoI construct digestion.
Dimer formation occurred during the Q column purification step.
Spin column purification and / or magnetic bead technology and reversed-phase purification are frequently applied.
Phenol / chloroform extraction and column purification are not necessary.
Combined treatment of coking-plant effluent by biological and carbon column purification.
See also
In the dark overnight, followed by column purification and NaOH treatment.
Further steps may include removal of the protease e.g. vía affinity column purification.
A second NAP-5 column purification of the product fraction was performed before use.
After filtration and evaporation of the solvent, column purification gave the title compound.
The column purification can be done on a column C8 or again on a column C18.
Table 11 shows the virus titer and recovery before and after the column purification.
Fig . 8 shows efficiency of Exo I treatment followed by column purification for different concentrations of construct.
The E. coli produced recombinant TcdA protein was purified by a series of column purification.
Concentration of the solvent and column purification ( 5 % ethyl acetate in hexane ) gave the product.
Ascites fluid was filtered and applied to a protein A monoclonal antibody column purification system ( MAPs ).
An additional type of column purification is taught by Potter, et al ., Cancer Lett.
Fourth, self-transposition products can be removed by column purification.
Column purification yielded homogeneous WOC-8 as determined by HPLC and TLC.
This experiment was carried through 3 rounds of column purification and 40 colonies were picked.
Step 4, HA Column Purification This step was only performed for the serotype 4 conjugate.
The average yield of this complexation reaction after column purification was found to be > 80 %.
Flash column purification ( 20 % ethyl acetate in hexane ) provided the title compound.
Unfortunately, only 23 % of virus was recovered after the double column purification process.
Each column purification is run at 4°C.
RFVIIa - ( CTP ) 2 is purified and activated by an ion exchange column purification process.
Typically, the 3H1 polypeptide ( s ) is coupled to an affinity matrix for affinity column purification.
FIG . 4, Checking the presence of DNAs in the column purification products.
RIP-std-Q represents immunoprecipitation followed by Qiagen column purification.
FIG . 4, Monitoring the presence of DNA in the column purification products.
The supernatant was filtered through a 0.45 µm membrane before column purification.
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