Examples of 'column volume' in a sentence
Meaning of "column volume"
Column volume: Refers to the amount of space occupied by a column, often used in the context of chromatography or chemistry
How to use "column volume" in a sentence
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column volume
The fractions are volume fractions of column volume.
Third column volume fractions were collected through out the gradient.
The difference comes from the displacement of a water column volume.
A fixed bed results in a minimal column volume and economizes on water consumption.
Column volume is abbreviated herein as CV.
The protein elutes with the second column volume of the eluant.
The third column volume contained the reductase activity which was collected.
The rHPC eluted at the beginning of the second column volume using this elution buffer.
The solvent column volume is also proportional to the cell size in use.
The matrix was eluted with a total of 5 column volume of elution buffer.
More preferably 1 column volume of equilibration buffer is passed over the column.
The following column operating parameters are appropriate for a column volume of 10 liters.
Typically the solvent column volume is determined from the sample cell size.
The unbound protein impurities are washed with at least 5 column volume of starting buffer.
A column volume is defined, as the volume of solvent needed to traverse the column.
See also
This was followed by a 2 column volume flush with equilibration buffer.
The following abbreviations have been used in the specification and examples, CV column volume.
Preferably, a fixed percentage of the entire column volume will be refreshed on a daily basis.
The S1 subunit emerged as a sharp peak at the column volume.
Load sample and follow with 1 column volume 10mM potassium phosphate.
After binding the protein, the column was washed with up to 5 column volume of PBS.
A column volume of CHCl 3 is added ( to eliminate the pigments ) until a translucent eluate is obtained.
Following the load, the column was washed with one column volume of equilibration buffer.
After equilibration, 18 column volume of immunoglobulin containing solution can be loaded into the column.
The agarose pellet was then re-suspended with one column volume of 1X flag binding buffer.
Fractions of 0.1 column volume are collected and assayed for the GRF analog.
A sample volume of about 1 % of total column volume is applied.
If ethanol is used, a column volume of 100 % isopropanol should also preferably be used afterward.
The same phosphate buffer was used to wash the column for 4-5 column volume.
LFA-3 elutes in less than about one column volume and is neutralized immediately.
The volumes will generally be in the range of 10 to 30 times the column volume.
The column was washed with 1 column volume of ATP Start buffer with 1 M NaCl.
The columns were then washed with 1 volume of H2O and 1 column volume of hexane.
TFPI was eluted in a 25 column volume gradient up to 20 mg / ml of polyphosphate.
Non-adsorbed proteins were removed by washing out with one column volume of TD1.
During the last elution step, one column volume of 0.85 M buffer was applied.
In some embodiments, the total flow-through volume is about 100 times of the column volume.
The column was washed with one column volume of 0.5 molar NaCl.
In other embodiments, the total flow-through volume is about 500 times of the column volume.
Thrombin passes through the column and elutes during a 2-6 column volume wash with 20 mM equilibration buffer.
The volume of the load should not exceed 5 % of the total TOYOPEARL HW65S column volume.
After loading, the reverse phase column was washed with one column volume of 0.2 M acetic acid.
The volume of the load should not exceed 25 % of the SEPHADEX G25 column volume.
USA slurried in water to give a packed column volume of 0.24 mL.
The gradient then stepped to 100 % Buffer B for one column volume.
The coloured substances were then eluted from the columns using 1 column volume 0.1 % HCl in methanol.
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