Examples of 'competent cells' in a sentence
Meaning of "competent cells"
A term commonly used in molecular biology and genetic engineering to describe cells that have been modified or treated to have an increased ability to uptake and incorporate foreign DNA. Competent cells are often used for the purpose of introducing exogenous DNA into a host organism
How to use "competent cells" in a sentence
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competent cells
Competent cells for electroporation were prepared as follows.
Sebocytes are the competent cells of the sebaceous gland.
Competent cells of both strains were prepared.
Preparation of competent cells and electroporation.
Competent cells may be obtained from any of a variety of sources.
Two uL of the ligation mix were added to the competent cells.
Electroporation competent cells were prepared from these cells.
The ligation mixture was used directly to transform competent cells.
Tubes of competent cells were thawed on ice.
The ligation mixture was then used to transform chemical competent cells.
Competent cells are thawed on ice.
Cells in flocs are separable from the in competent cells by differential sedimentation.
The competent cells are left on ice until ready to use.
Genetic vaccines are expressed upon administration to a patient an uptake by competent cells.
Competent cells can be made by inducing artificial competence in the laboratory.
See also
The resulting vectors were introduced into competent cells for cloning.
Competent cells are then prepared from the host cells containing the vector.
The reaction is placed on ice and transformed into competent cells.
Connections were then used to transform competent cells in order to confirm the cloning.
The two fragments are ligated and transformed into competent cells.
Both are electroporated into competent cells to allow recombination and gap repair of the plasmid.
The recombinant circles thus formed were used to transform an aliquot of competent cells.
The invention further relates to prokaryotic competent cells produced by such a method.
Genetic vaccines are expressed upon administration to a patient and uptake by competent cells.
Electroporation competent cells are able to take up DNA during electroporation.
By electroporation of competent cells.
Preparation of competent cells Electroporation competent cells were prepared by standard methods.
This new ensemble of stochastic sequences can then be used to transform competent cells.
Electroporation or chemical transformation of competent cells with purified DNA may also be used.
Use of SOC maximizes the transformation efficiency of competent cells.
The chemically competent cells that were used for this manipulation were obtained from Stratagene Cat.
Results are from five experiments that used the same batches of competent cells and DNAs.
Competent cells were prepared as described in BioRads Gene Pulser manual.
Cells that are able to take up the DNA are called competent cells.
Escherichia coli DH5a competent cells were transformed.
Competent cells were prepared by the CaCl2 procedure.
Pluvialis is introduced to 1 ml of the competent cells.
These are transformed into competent cells and uracil-containing phagemids are grown.
Ligated plasmids were transformed into DH5aTM competent cells.
Rubidium chloride transformation for competent cells is arguably the compound 's most abundant use.
The ligation product was used to transform DH5a competent cells.
Competent cells of DH5alpha were purchased from Toyobo.
For most transformations, homemade competent cells were used.
Coli DH1 competent cells were transformed with the mixture.
The plasmid was subsequently used to transform DH5a competent cells.
DH5alfa competent cells were transformed directly with ligation mixtures of the samples.
Of a different origin from the baculovirus, which is functional in competent cells.
OxLDL promotes inflammation also in immune competent cells from atherosclerotic lesions17.
Half of this volume was used for electroporation together with 50 µl competent cells.
The ligated product was transformed into competent cells of Escherichia coli DH5a strains.
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This conversion must be performed by a competent person
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Examples of using Cells
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For cells or batteries packed with equipment
They were beaten in the cells or in the courtyard
The cells we want can not be tainted
