Examples of 'cotransfected' in a sentence
Meaning of "cotransfected"
Cotransfected is the past tense of the verb cotransfect, which refers to the simultaneous transfection of multiple genes or genetic material into a cell or organism
Show more definitions
- simple past tense and past participle of cotransfect
How to use "cotransfected" in a sentence
Basic
Advanced
cotransfected
The cells were cotransfected with pLacZNorm for normalization of transfection efficiency.
The same signal was also found in lysates of cells cotransfected with inactive neurotrypsin.
DsRed was cotransfected as a transfection control.
The transposase expression vector and the corresponding transposon vector are cotransfected into human HeLa cells.
An insect cell is cotransfected with the resulting transfer vector and a baculovirus DNA.
The PpLuc Signal was corrected for transfection efficiency by the signal of cotransfected RrLuc.
The various indicated promoter constructs were cotransfected with a luciferase plasmid as an internal standard.
It was cotransfected with wild-type virus into an avian hepatoma cell line.
A plasmid containing the AAV genes is cotransfected with the novel construct.
As an internal control for transfection efficiency a constitutive renilla luciferase reporter ( promega ) was cotransfected.
In such a case, vectors are cotransfected into a host cell.
For normalizing the transfection efficiency, a GFP expression plasmid was cotransfected.
The enzyme activity of the cotransfected cells was 30x higher than that of the negative controls.
Expression of YFP was studied under the effect of simultaneously cotransfected dsRNA with sequence homology.
The Sf9 cells were cotransfected with these DNAs by liposome-mediated transfection.
See also
Coprecipitation of Notch was detected in Delta immunoprecipitates from cotransfected cells and embryos.
An Fc expression cassette is cotransfected with BDDFVIII-Fc to generate the BDD FVIIIFc monomer -.
In the latter case, the two expression vectors may be cotransfected into a cell.
Time lapse imaging of cotransfected HeLa cells expressing GFP-PCNA and anti-GFP chromobody.
This induction was reduced in cells cotransfected with RORa1.
The recombinant vector is then cotransfected into Lepidoptera cells with DNA from a wild-type baculovirus.
In this case, the host cell may be simultaneously cotransfected with both vectors.
C6 cells were cotransfected with the above described adapter plasmid pAdApt35.
In the latter case, the two nucleic acid molecules may be cotransfected into a cell.
Staining in FL1 of protein encoded by the cotransfected GFP expression plasmid illustrates expression efficiency.
Western blot data revealed complete cleavage of the cscFc linkers when cotransfected with PC5.
After the incubation of the cotransfected cells in step ( d ), the maintenance medium is discarded.
In this assay, the substrate, HCV protease and a reporter gene are cotransfected into COS cells.
S02 targeting MegaTAL was cotransfected with an independently synthesized mRNA species encoding Trex2.
Luciferase was normalized to protein since GPCR induced expression of the cotransfected B-galactosidase.
The term " transfer vector " refers to a vector cotransfected into an insect cell with a wild-type baculovirus.
In order to improve experimental accuracy the plasmid pRL-CMV ( Promega ) was cotransfected.
The cells were cotransfected with pSV2Neo so that individual clones could be selected with G418.
The murine T-lymphocyte cell line B3Z was transiently cotransfected with two plasmids.
Cells were cotransfected with an SV40 promoter-B galactosidase expression vector to control for transfection efficiency.
Immunohistochemistry with anti-FLAG antibody in cotransfected cells with pEYFP-N1.
The vectors were cotransfected into the rat myeloma YB2/0 Kilmartin et al.
Portions of cell lysates were assayed for B-galactosidase activity from the cotransfected pRSV-B-gal plasmid.
Luciferase activity was normalized against cotransfected Renilla-luc activity using a commercial kit ( Clonetech ).
To monitor successful transfection, IVT RNA encoding infrared fluorescent protein ( iRFP ) was cotransfected.
A large molar excess of these clones is cotransfected with a sub-infectious dose of Sb-1 genomic DNA.
NIH 3T3 fibroblasts were transfected with Jab1 or control vector and cotransfected with EGFP.
To test this hypothesis, CHO cells were cotransfected with KVLQT1 and human minK ( hminK ) cDNAs.
These were cotransfected with firefly and Renilla luciferase expression plasmids into 293T cells.
The heterodimers are purified from cell lines cotransfected with the appropriate alpha ( a ) and beta ( B ) components.
The plasmid was cotransfected into NIH 3T3 cells by the lipofection procedure.
RIU, relative light units ( normalized against cotransfected Renilla luciferase ).
Cells may be cotransfected with the pR2Luc plasmid and an siRNA against R2.
A fixed amount of 0.5 ug IVT RNA coding iRFP was cotransfected to monitor lipofection success.
Vectors are cotransfected to HEK-293 cells and cells are treated with test compounds.
