Examples of 'crrna' in a sentence
Meaning of "crrna"
crrna (adjective) - Pertaining to a specific type of RNA molecule used in genetic research, particularly in CRISPR technology
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- Initialism of CRISPR RNA.
- Alternative form of crRNA
How to use "crrna" in a sentence
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crrna
The tracrRNA can remain hybridized to the crRNA.
The nucleic acid tag can comprise a crRNA.
The crRNA must bind only where editing is desired.
Sequences for the mature crRNA and tracrRNA are shown.
Target recognition specificity comes from the crRNA design.
Sequences that are removed post crRNA maturation are colored gray.
The crRNA and the corresponding tracrRNA hybridize to form a gRNA.
A subject gRNA can comprise any corresponding crRNA and tracrRNA pair.
Northern blot analysis of crRNA and tracrRNA expression in mammalian cells.
A gRNA can comprise a nucleic acid sequence encoding a crRNA and a tracrRNA.
Cpfl crRNA from different species may exhibit different degrees of tolerance for various sequence modification.
The guide RNA may be a dualRNA comprising a crRNA and a tracrRNA.
The crRNA may hybridize with a target DNA.
Total reads retrieved for the putative crRNA are calculated from the SAM file of alignments.
The crRNA was chemically synthesized by Sigma-Aldrich.
See also
In certain embodiments, the mature crRNA preferably comprises a single stem loop.
A crRNA can refer to the mature form of the spacer-repeat unit.
In some aspects, the tracRNA sequence is homologous to a crRNA over 6 contiguous nucleotides.
As such, each crRNA can be said to have a corresponding tracrRNA.
In one embodiment, the gRNA comprises a nucleic acid sequence encoding a crRNA and a tracrRNA.
The crRNA additionally provides the single stranded DNA-targeting segment.
Cas genes can be involved in the biogenesis and / or the interference stages of crRNA function.
The crRNA and the corresponding tracrRNA ( as a corresponding pair ) hybridize to form a gRNA.
The CRISPR/Cas9 system requires only the redesign of the crRNA to change target specificity.
The tracrRNA of the complex can hybridize to a crRNA repeat in the pre-crRNA array.
By contrast Cas9 requires both crRNA and a transactivating crRNA tracrRNA.
The tracrRNA is required for crRNA maturation from a primary transcript encoding multiple pre-crRNAs.
Figure 9A-B illustrates the results of a Northern blot analysis of crRNA processing in mammalian cells.
The crRNA additionally provides the single stranded DNA-targeting segment that hybridizes to a target sequence.
In some aspects, the CRISPR repeat sequence is homologous to a crRNA over 6 contiguous nucleotides.
Wherein the crRNA contains a target-specific nucleotide region complementary to a region of the universal primer, optionally.
Cmr proteins form a complex with mature crRNA to site-specifically target and cleave RNA.
Such crRNA allow increasing the specificity of the Cas9 activity.
Class 2 CRISPR systems use a single Cas protein with a crRNA.
Sequences used for crRNA template assembly are shown in Table 7.
As detailed above, the guide RNA of type V CRISPR / Cas systems comprises only crRNA.
In one embodiment, the crRNA and the tracrRNA are expressed as separate RNA transcripts.
In other embodiments, the guide RNA of type V CRISPR / Cas systems can comprise only crRNA.
In some aspects, the crRNA is expressed from a CRISPR / Cas system within the microorganism.
For example, the guide RNA of type II CRISPR / Cas systems can comprise crRNA and tracrRNA.
As observed for crRNA processing within two other CRISPR / Cas subtypes Brouns et al.
The recognition sites are defined by 20-nucleotide target sequence within an associated crRNA transcript.
The corresponding crRNA is expressed using a PCR fragment containing a U6 promoter fused to the crRNA sequence.
Figure 6 shows DNA binding and cleavage analysis of Cas9-Chis protein lacking crRNA.
The crRNA additionally provides the single-stranded DNA-targeting segment that hybridizes to a CRISPR RNA recognition sequence.
The 5 ' handle is required for the crRNA to be included in the Cmr complex.
These crRNA snippets are used to spot " enemy " presence.
The sequence and length of S . pyogenes crRNA was determined by deep sequencing analysis ( 2 ).
Thus, a crRNA and a tracrRNA ( as a corresponding pair ) hybridize to form a gRNA.
Figure 12A-B shows the results of a Northern blot analysis of crRNA processing in mammalian cells.
