Examples of 'crude enzyme' in a sentence
Meaning of "crude enzyme"
Crude enzyme: an enzyme that has not been purified or processed, often extracted directly from its natural source for use in various applications or experiments
How to use "crude enzyme" in a sentence
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crude enzyme
Alternatively the crude enzyme preparation was lyophilised.
Crude enzyme solution was obtained in the following way.
Subsequent filtration gave a crude enzyme solution as filtrate.
The crude enzyme solution is concentrated if necessary.
The resulting supernatant was used as a crude enzyme solution.
A crude enzyme solution was obtained as a supernatant by centrifugal separation.
The supernatant is used as the crude enzyme extract.
The crude enzyme is then purified by known methods.
Preparation of crude enzyme extracts.
Crude enzyme extract of wheat.
Assays were performed on crude enzyme extracts prepared as described below.
The supernatant can be directly used as a crude enzyme solution.
The crude enzyme sample of iNOS was prepared by the following procedure.
Of course the intact culture can be used as a crude enzyme.
The lysate was used as a crude enzyme solution to measure the galactose isomerization.
See also
The resultant culture can be also used intact as a crude enzyme.
These crude enzyme mixtures may include the organism producing the enzyme.
This fraction was used in the subsequent experiments as a crude enzyme preparation.
Although crude enzyme preparations can also be used it is preferable to use purified enzyme.
Extracts from cells can be used in the invention as a crude enzyme solution.
The crude enzyme solution can then be purified by conventional purification methods for proteins.
The supernatant of the resultant suspension is obtained by centrifugation as a crude enzyme solution.
Endoxylanase and the crude enzyme preparation were in this model not as effective as AXDA.
The cell extracts so prepared were separately centrifuged to produce aqueous crude enzyme solutions.
The crude enzyme was thus purified to obtain alkaline pullulanase A.
Enzyme preparations extracted from cells can be used in the invention as a crude enzyme solution.
Again, also less purified crude enzyme preparations may be used.
One can extract enzymes from cells and use the extract as crude enzyme liquid.
The crude enzyme sample of E-cNOS was prepared by the following procedure.
The procedure for quantifying protein concentration in the crude enzyme solution is shown below.
The crude enzyme sample of N-cNOS was prepared by the following procedure.
The supernatant was then filtered through glass wool with the filtrate being the crude enzyme.
The obtained filtrate, that is, a crude enzyme solution of transglutaminase is cooled if necessary.
After centrifuging, the supernatant was used as a crude enzyme solution.
Supernatant was collected as a crude enzyme solution, and provided for further purification.
Generally, the present invention is feasible with a crude enzyme.
The crude enzyme was processed according to a conventional method to prepare ethanol-dried powder.
In some circumstances, it may be desirable to used a relatively crude enzyme preparation.
By reacting the crude enzyme solution on sucrose, the antitumor dextran can be produced.
Next, the dialysis inner solution was lyophilized to obtain crude enzyme powder.
First, a crude enzyme solution was extracted from barley seeds by the following method.
Solids in the solution are then removed by centrifugation, to obtain a crude enzyme solution.
After removal of the residue, these crude enzyme solutions were analyzed by SDS-polyacrylamide electrophoresis.
After centrifuging, the supernatant was collected and referred to as a crude enzyme solution.
Using the crude enzyme solution thus prepared, transketolase activity was measured in the following manner.
Comparison of the purified a-glucosltransferase and crude enzyme in the formation of the branched a-glucan.
A crude enzyme sample shown in Table 6 was obtained.
The inosine-guanosine kinase activity of the crude enzyme extract was measured by the following method.
Both the cell-free supernatant and the culture broth can be used as a crude enzyme preparation.
The crude enzyme samples shown in Table 5 were obtained.
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