Examples of 'culture flask' in a sentence
Meaning of "culture flask"
A culture flask is a type of laboratory equipment used for the cultivation and growth of microorganisms or cells. It usually consists of a glass container with a removable cap or stopper, providing a sterile environment for the organisms to grow and multiply
How to use "culture flask" in a sentence
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culture flask
The cell suspension is transferred to a culture flask.
A cell culture flask is preferred.
Detachment of cells from culture flask.
The culture flask in which the cells are cultured is prepared as follows.
Monocytes were depleted by adherence to culture flask.
The number of cells in each culture flask is determined simultaneously.
Monocytes and macrophages can be removed by adherence to the culture flask.
The number of cells added to a cell culture flask is proportional to the área.
The three cultures are handled separately in each their culture flask.
Cells are removed from the culture flask and a cell pellet is prepared by centrifugation.
The cells were allowed to adhere to the culture flask overnight.
The cells in the culture flask were then collected and injected into a mouse abdominal cavity.
The appropriate amount of cell solution is placed in the new culture flask.
For example, plating of PBMCs in a tissue culture flask permits adherence of monocytes.
The organ pieces were rinsed with PBS and pieces were pipetted in a culture flask.
See also
These cells are then dissociated from their culture flask in a solution of trypsin / EDTA.
To harvest the cells, the medium was aspirated from the culture flask.
The contents of each culture flask are then suspended in 10 ml of PBS.
Then, only mesenchymal stem cells attached to the culture flask were collected.
Finally, the culture flask with its contents was placed on an orbital shaker overnight at 4°C.
Forty milliliters of the suspension was put in a tissue culture flask manufactured by Falcon No.
Subsequently, the cell suspension with all reagents is transferred to a culture flask.
Cells were harvested by rinsing the culture flask with a 10 ml volume of DMEM+FBS medium.
The support and the filter then drop into the plate well and / or the culture flask.
A preferred culture apparatus is a culture flask, and a more preferred culture apparatus is a T-225 culture flask.
Subsequently, the lymphocytes were incubated overnight in a polystyrene culture flask in order to remove monocytes.
HUES7 human embryonic stem cells were cultured on gelatin coated tissue culture flask.
Exponentially growing cells were detached from culture flask using 0.5 mM PBS / EDTA buffer.
The pellet was resuspended in culture medium and transferred to a new T25 culture flask.
Cell solutions were transferred to a culture flask and incubated at 37 °C in a humidified 5 % CO2 atmosphere.
A total of 15 explants were set up in each culture flask.
The cells were detached from the culture flask with 3-4ml of a 5mM EDTA / PBS solution.
Five mL of autoclaved 50 % glucose were then added into each culture flask.
Cells were liberated from the T175 culture flask for passage and harvesting using Versene ( Invitrogen ).
The cell suspension was then transferred into a 25 cm ² tissue culture flask.
In parallel, a control was provided by one tissue culture flask without any addition.
In other embodiments, 25 micromolar CLQ is added to the culture flask.
As a vessel, a plate culture vessel or a roller culture flask for example can be used.
PBS is removed and 2 ml Trypsin-EDTA is added to the culture flask.
The attached cells were selected by gently agitating the culture flask to remove non-adherent cells.
These cells are finally resuspended in Amniomax culture medium in a 25 cm2 culture flask.
Alpha screen, Cells are washed in the culture flask once with PBS.
An enzyme solution (in PBS) is added to detach the cells from the culture flask.
For example, in the case of a small-scale culture, a flat culture flask may be preferably used.
These cells were trypsinized, counted and seeded into a T75 culture flask.
Threshold determination Cells are transferred into a 750 ml tissue culture flask and grown overnight culture at 32 °C.
One million cells of ANCs from bone marrow were seeded on T25 cell culture flask ( Nunc ).
Figure 5 is a front elevational showing of the tissue culture flask of Figure 1.
Inoculum for the experiment was grown in a 162 cm2 tissue culture flask.
Figure 2 is a side elevational showing of the tissue culture flask of Figure 1.
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