Examples of 'datp' in a sentence
Meaning of "datp"
dATP is a type of nucleotide that is one of the building blocks of DNA
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- Initialism of deoxyadenosine triphosphate.
How to use "datp" in a sentence
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datp
Gap filling nucleotides were dATP and dGTP.
The dATP was shown to enhance the rate of cleavage by the polymerase.
The unreacted dATP is eluted first.
Reactions were performed with both dATP and dCTP.
Luciferase can hydrolyze dATP directly with concomitant release of a photon.
Utilising enzyme variants with reduced utilisation of dATP as a substrate.
ATP and dATP are two types of adenine nucleotides.
This is explained by the substitution of dITP for the absent dATP.
ATP is a ribonucleotide while dATP is a deoxyribonucleotide.
The activity site can bind either ATP or dATP.
ATP consists of a ribose sugar while dATP consists of a deoxyribose sugar.
Secondly its formation requires the hydrolysis of a high energy bond of ATP or dATP.
One type of control sample contained dATP instead of dATPaS in the SDA reaction.
However, good results were obtained in the absence of dATP.
Thus, a labeled dATP is a good choice in this example.
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Thus, the frequency of mutation increased as the dATP concentration decreased.
These dATP molecules then inhibit ribonucleotide reductase, which prevents of DNA synthesis.
As the phosphate donor, there are ATP or dATP.
Alternatively, Epoxy dATP can be use to inactivate the enzyme.
FISH detection was carried out with a Mus81 cDNA probe labelled with biotinylated dATP.
The electrophoresis of the fragments labelled with S35 dATP was carried out on a ureapolyacrylamide gel.
The method of claim 3, where said cofactor is dATP.
To increase the signal-to-noise ratio, the natural dATP has been replaced by dATPaS.
Suitable sized fragments of 9-23 bp were partially filled in the presence of dGTP and dATP.
However, the use of the base-modified analogs of dATP and dTTP is preferred.
Without dATP addition " apocyclin-A was unable to induce DEVD-ase activity in the same experimental conditions.
The signal-to-noise ratio was increased by the use of a-thio dATP in the system.
Similarly, the Y-phosphate in the dATP may be radio-labeled for monitoring the A-tailing step.
All the sequencing reactions were carried out with 35 S-labelled dATP.
The first-strand cDNA is then tailed with dATP and terminal deoxynucleotide transferase ( Gibco / BRL ).
For example, in Step 2 above the labelled nucleotide could be dATP.
And dATP as recommended by the supplier ( BRL Labs ).
The top strand of each target site was labeled with polynucleotide kinase and Y-32P dATP.
Stabilisers were tested on dATP ( alpha 35S ) nucleotide solutions.
Clones containing reverse transcriptase ( RT ) activity are selected and sequenced using ³ ² P dATP.
In the following data, the nucleotide used in testing was ³ ⁵ S dATP at a concentration of 10 mCi / ml.
The FPET cDNA is then tailed with Terminal Transferase ( TdT ) and dATP.
Sequencing was carried out using [ 33P ] dATP and the fmol kit ( Promega ) as described above.
The cDNA probes are prepared by reverse radioactive transcription ( alpha dATP 33 p-Amersham ).
The polymerase domains and the dATP and dTTP are incubated at a fixed temperature ( generally about 70°C ).
A further experiment was carried out to tes stabilisers on dATP ( Alpha 35S ).
Human DNA polymerase B exhibited a steady state km of 3.33 µM for dATP.
