Examples of 'dctp' in a sentence
Meaning of "dctp"
dctp: An acronym for Deoxycytidine triphosphate, a molecule that plays a crucial role in DNA synthesis
How to use "dctp" in a sentence
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dctp
Gap filling nucleotides were dCTP and dTTP.
The dCTP concentration in the reaction is thus a limiting substrate.
The part representing dCTP is indicated by black color.
Reactions were performed with both dATP and dCTP.
The dCTP molecules will be understood to be the same species as each other.
The reaction was stopped by dCTP addition.
The enzyme dCTP deaminase is subject to feedback inhibition by TTP.
This activity was insensitive to inhibition by other nucleosides and dCTP.
The labeled fragment was separated from unincorporated dCTP by standard chromatographic methods.
The CTP peak is made up of both CTP and dCTP.
The fourth key enzyme is dCTP deaminase, which catalyzes the deamination of dCTP to dUTP.
Aptamers may include modified dUTP and dCTP residues.
Second, dFdCTP competes with dCTP for incorporation into DNA self-potentiation.
The enzyme dCMP deaminase is subject to allosteric activation by dCTP and inhibition by TTP.
Table 5 shows the increased incorporation of 32P dCTP into hybridisation mixtures including the amplification oligonucleotide 11.
See also
High-performance liquid chromatography dCTP.
Thus, the reduced intracellular concentration of dCTP potentiates the incorporation of dFdCTP into DNA.
In the following examples these abbreviations may be employed, dCTP.
Preferential effect of ADA on dCTP pools in PHA-stimulated PBMC.
For example, a mixture of nucleotides can include several dCTP molecules.
Probe was radio-labeled with Y32 - P dCTP using a random primer labeling kit from Invitrogen.
The same overall method was applied to dGTP, dCTP and dTTP.
To the solution were added dATP, dTTP, dCTP and dGTP each to a concentration of 0.5 mM.
Thereby nucleotides are used which comprise differentially labeled dCTP and / or dTTP.
This resulted in over 60 % of the radiolabelled dCTP being incorporated into the PCR products.
The dNTP 's that must be added during probe extension are dCTP and dTTP.
In these standard reactions 20 % of the dCTP is replaced by Cy-dye labelled dCTP.
However, in the long slender forms isolated from mice also the dCTP pool is low.
For 5 ' RACE, a homopolymeric dCTP tail was added to the 3 ' end of the cDNA.
In some embodiments, the deaminase is a Pyrococcus horikoshii dCTP deaminase.
This filter was hybridized with the 32P dCTP labeled 2.3 kb PstI-DraI deletion fragment as probe.
In some aspects, the deaminase is Pyrococcus horikoshii dCTP deaminase.
From these nucleotides, the dCTP is labelled by radioactive phosphorus ( 32 P ).
Extension products were radioactively labeled by including 32P dCTP in the reaction mixture.
From these nucleotides, the dCTP is labelled with radioactive phosphorus ( 32 P ).
In case the cytosine in the original DNA was methylated, then dCTP will be incorporated.
Produced by, dctp TV, Düsseldorf.
PDH51 was digested with XbaI and the ends were partially filled with dCTP and dTTP.
The assay is calibrated using a known amount of the labeled dCTP solution ( omitting the washes ).
The cDNA clones complementary to specific mRNAs were radioactively labeled by primer extension with 32P dCTP.
Second strand synthesis was performed in the presence of 32p - dCTP to label the cDNA.
The amplified promotors were treated with T4 DNA polymerase in the presence of dCTP.
The CYP7 transgene specific probe is labeled with 32P dCTP by nick translation.
Deoxynucleotides may be selected from, but not limited to, dGTP, dATP, dTTP and dCTP.
The radioactive concentration of the dCTP was 10 mCi / ml.
The gap-filling nucleotide was dCTP.
CDNA probes are labelled by nick translation using 35S dCTP as substrate.
The probe corresponded to the entire clone 97 labeled with alpha 32P dCTP.
One can generate a single base 3 ′ C overhang using Klenow ( exo - ) and dCTP.
