Examples of 'definitive endoderm' in a sentence
Meaning of "definitive endoderm"
definitive endoderm: In biology, it refers to the innermost layer of cells in the gastrula stage of an embryo, from which the digestive and respiratory system develop
How to use "definitive endoderm" in a sentence
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definitive endoderm
Expression of the definitive endoderm markers was detected by immunohistochemistry.
This protocol resulted in significant upregulation of definitive endoderm markers.
Human definitive endoderm cells were produced as described in the foregoing Examples.
The cells were then incubated in definitive endoderm differentiating medium as above.
The intermediate stage in this process is the formation of definitive endoderm.
Such processes include providing definitive endoderm cells with retinoic acid.
Most of above cell types are epithelialized as compared to mesenchymal definitive endoderm cells.
In some embodiments, definitive endoderm cells are grown with serum replacement.
Formation of the pancreas arises from the differentiation of definitive endoderm into pancreatic endoderm.
A definitive endoderm cell is a lineage of a mesendoderm cell, one of its precursors.
These data suggest that the cells retain the capacity to differentiate into definitive endoderm.
Definitive endoderm expression was equivalent between cells grown on the different micro-carriers.
Step 1 was conducted over three days to generate definitive endoderm cells.
In some processes, definitive endoderm cells are grown without serum or with serum replacement.
In a preferred method, however, the cells are directed to differentiate primarily into definitive endoderm.
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In some processes for producing definitive endoderm cells, hESCs are maintained on a feeder layer.
Figure 15 shows the induction of endocrine markers using an alternative method to induce definitive endoderm.
The cells were efficiently differentiated into definitive endoderm regardless of the micro-carrier type.
Percent of definitive endoderm marker CXCR4 positive cells is stated in upper right corner.
Differentiating the pluripotent stem cells on the microcarriers into definitive endoderm cells ; and.
Such processes include providing definitive endoderm cells with an FGF-family growth factor.
This demonstrates that CXCR4 is also a marker of definitive endoderm.
Another marker of definitive endoderm is the CXCR4 gene.
Expression of the transcription factor SOX17 is considered indicative of definitive endoderm differentiation.
Differentiation of hESC to definitive endoderm by 3D culture methods: towards adult hepatocytes.
For RT-PCR analysis, cells were analyzed for expression of selected panel of definitive endoderm markers.
Definitive endoderm cell populations and methods of production thereof are also described in U. S.
CXCR4 is regarded as a marker for definitive endoderm cells.
The negative control for definitive endoderm production ( NF ) received 2 % FBS / RPMI with no added activin A.
Next these H1 cells on the micro-carriers were differentiated to definitive endoderm.
In still other processes, definitive endoderm cells are grown in the presence of B27.
Here FACS plots display the percentage of cells expressing the definitive endoderm marker CXCR4.
The cells were differentiated to definitive endoderm as above but with DMEM/F12 instead of RMPI.
For both H-lines, addition of WNT-3a resulted in significant upregulation of definitive endoderm markers.
FACS analysis was conducted for the definitive endoderm cell surface marker CXCR4 ( Figure 28 ).
In addition, the CXCR4 - fraction contained very little gene expression for these definitive endoderm markers.
Both cell population were then differentiated to definitive endoderm on a rocking platform at 37°C.
Each of the eight cell lines differentiated to SOX17/CXCR4-positive definitive endoderm cells.
Figure 30 depicts the gene expression of definitive endoderm markers by real-time PCR.
Figure 28, Differentiation of H1 cells on micro-carriers to definitive endoderm.
Figure 23 depicts the real-time PCR analysis of definitive endoderm markers at day 4 of differentiation.
Figure 47, panels a and b depicts the gene expression of definitive endoderm markers at day 5.
Further this SOX17 expression predominantly represented definitive endoderm as opposed to extra-embryonic endoderm.
FACS analysis of cell surface expression of CXCR4, definitive endoderm marker.
In this example, Sox17 expression is indicative of definitive endoderm differentiation.
A PDX-1 positive pancreatic endoderm cell is a lineage of a definitive endoderm cell, one of its precursors.
RA and FGF-10 may be both provided to the PDX1-negative definitive endoderm cell culture.
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Examples of using Endoderm
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It marks cells destined to be mesoderm and endoderm
The endoderm allows them to develop true tissue
Panel j shows extra embryonic endoderm markers
