Examples of 'deglycosylation' in a sentence
Meaning of "deglycosylation"
deglycosylation (noun) - The process of removing glycosyl groups from molecules, often used in biochemistry and molecular biology to modify proteins or other substances
Show more definitions
- The removal of the sugar entity from a glycogen (but especially from a glycoprotein)
How to use "deglycosylation" in a sentence
Basic
Advanced
deglycosylation
This deglycosylation approach results in faster clearance.
Other studies report loss of activity following deglycosylation and that disulfide bonds are additionally required.
Deglycosylation and removal of sialic acid.
Molecular size and deglycosylation of the expressed phytase.
Deglycosylation is a technique for enzymatic digestion using glycosidase.
The polyclonal antiserum recognized CEA before and after deglycosylation.
Chemical deglycosylation requires exposure to trifluoromethanesulfonic acid or an equivalent compound.
Panel A illustrates the deglycosylation of lactoferrin.
Deglycosylation enzymes are also contemplated.
Commercially available kits are available for removing post-translational modifications, including deglycosylation.
A deglycosylation step was first performed.
Additionally, multiple steps of glycosylation / deglycosylation occur in the Golgi.
Chemical deglycosylation techniques are applicable.
Immunoprecipitated or affinity purified samples were blotted after N-deglycosylation or without deglycosylation.
Data do not indicate deglycosylation or polypeptide changes with storage time.
See also
Western blot analysis by using p35 specific antibody and deglycosylation experiments Fig.
Deglycosylation of the enzymes.
This was incubated overnight and the deglycosylation analysed with SDS-PAGE.
Deglycosylation can be accomplished using one or more glycosidases known in the art.
In some embodiments, the first step of deglycosylation removes at least one N-linked glycan.
However deglycosylation of the enzyme does not affect the activity of the enzyme.
Asparagine is transformed into aspartic acid through endogenous deglycosylation of N-coupled sugar residues.
The enzymatic deglycosylation of the peptides was performed simultaneously with the digestion with the endoprotease.
The mass of the respective antibody chains was determined by ESI-MS after deglycosylation and reduction.
Deglycosylation experiments showed that these two bands are differentially glycosylated forms of the same protein.
The Gno glycoform was characterized by MALDI-TOF-MS to confirm the deglycosylation.
Chemical deglycosylation is further described by Hakimuddin et al.
N-glycosidase F was used for deglycosylation of proteins.
Deglycosylation was detected by Western blotting.
Rapid labeling protocol Upon completion of enzymatic deglycosylation with PNGase F,.
Similar effects of complete deglycosylation are obtained with the additional reproductive hormones LH and FSH.
Some full-length polypeptides have a molecular weight of about 50 kDa before deglycosylation and about 27 kDa thereafter.
Furthermore, enzymatic deglycosylation of the ovomucoid protein was performed.
Both photoprobes label a 55 kDa band that shifts to 45 kDa upon deglycosylation.
Enzymatic deglycosylation of purified u-PAR.
This aspect was specifically addressed by enzymatic deglycosylation of the purified product ( Fig 7 ).
The enzymatic deglycosylation was apparent from a decrease in molecular size by approximately 8 kDa.
Fig . 15 is a non-limiting example of an immunoblot analysis of the deglycosylation of TSG-6 in AM.
Chemical deglycosylation requires exposure of the protein to trifluoromethanesulfonic acid, or an equivalent compound.
This reduced migration is reversed by deglycosylation with N-Glycosidase F ( EMP-1 deglycosylated ).
Deglycosylation of huKS-lL2 was verified be size exclusion chromatography and on a urea gel.
It was then incubated overnight at 37°C and the deglycosylation analysed with SDS-PAGE.
Several methods of deglycosylation are available in common practice, both chemical and biological.
Deglycosylation was monitored by SDS-PAGE after reduction and carboxymethylation.
The first step of deglycosylation may remove at least one N-linked glycan.
Deglycosylation may have revealed hidden epitopes for the CM1 antibody.
The first step of deglycosylation may remove at least one a2,6-linked sialic acid residue.
Deglycosylation of recombinant a-Gal A may be accomplished in a number of ways.
Aglycosylation and deglycosylation are known to decrease the Tm of the CH2 domain by 10°C.
Deglycosylation was confirmed by SDS-PAGE.
