Examples of 'desalting column' in a sentence
Meaning of "desalting column"
desalting column - A desalting column is a device used in the purification process to remove salts from a mixture, usually applied in chemical or biochemical processes
How to use "desalting column" in a sentence
Basic
Advanced
desalting column
The modified antibody was fractionated using a polyacrylamide desalting column.
The desalting column must be well equilibrated and running ( zeroed ) at time of loading.
The active fraction was desalted with a desalting column to give a pure enzyme.
After deacetylation with 5 mg of hydroxylamine HCl, modified IgG was separeted by polyacrylamide desalting column.
The active fractions were desalted with a desalting column to give a purified enzyme.
The fluorescently conjugated heparin was eluted on an Amersham PD10 desalting column.
The reaction was desalted on a desalting column ( Pierce ) by using PBS and fractions were collected.
A column employing an ion exchange resin is preferable for the desalting column.
The reaction mixture was desalted over a Fast desalting column ( Pharmacia ) equilibrated with PBS.
The eluent is removed from the protein by buffer exchange on a desalting column.
Equilibrate the desalting column with 15 ml of coupling buffer.
After this the reaction mixture was transferred to the desalting column equilibrated with PBS.
Meanwhile pre-equilibrate a desalting column by washing it with 50 mL degassed 5mM EDTA / PBS buffer.
The modified antibody was then purified by SephadexG25 desalting column with Buffer A.
Supernatant was loaded onto a HiTrap desalting column to remove any remnant of Triton-X114.
See also
Then, Fab ' fragment was collected by using a desalting column.
The solution is desalted using a Sephadex PD-10 desalting column and the product eluant is lyophilized.
These enzymes also require cofactors and cosubstrates that can be removed by a PD-10 desalting column.
Unbound SULFO-TAG was removed using a Zeba ™ spin desalting column Thermo Scientific, cat no.
After filtration, sample was passed through 5ml Zeba ™ desalting column.
Excess CO was removed by passing through a desalting column inside a glove box.
The solution was then centrifuged and desalted on a GE Healthcare PD-10 desalting column.
The reaction mixture was passed through 0.5 mL desalting column twice eluting with PBS.
The radiolabeled polymer was purified using a PD-10 ( Pharmacia IKB Biotechnology ) desalting column.
After a further 2 h incubation, unincorporated biotin was removed with a desalting column ( Amersham Pharmacia ).
An alternative method is to remove the reducing reagents through a desalting column such as Sephadex G-25.
If present, sodium azide was removed using a 2 ml Zeba desalting column ( 7 K MWCO ).
You'll also be interested in:
Examples of using Desalting
Show more
Animation of desalting using gel filtration chromatography
Fully automatic conductivity measurement and desalting
The two methods of desalting are on a continuous basis
Examples of using Column
Show more
This column also captures those situations
This marks my final column in this magazine
A column matrix is a matrix with a single column
