Examples of 'diafiltration buffer' in a sentence
Meaning of "diafiltration buffer"
diafiltration buffer - a solution used in the process of diafiltration to separate and purify biomolecules in a sample
How to use "diafiltration buffer" in a sentence
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diafiltration buffer
The diafiltration buffer is prepared according to the final container albumin concentration.
The retentate is collected and additional diafiltration buffer is added to wash the membrane filter.
The diafiltered concentrate was mixed with hydroxylapatite ( Calbiochem ) equilibrated in diafiltration buffer.
The system was then rinsed with 40 mL of Diafiltration Buffer to ensure maximum product recovery.
The ultrafiltration unit was postwashed twice with 1.5 kg diafiltration buffer.
The use of a Tris-HCl diafiltration buffer with a pH of 8.5 but of unspecified concentration.
Preferably the reducing agent in the protein sample and in the diafiltration buffer is B-mercaptoethylamine.
Add 500ml of fresh diafiltration buffer to the reservoir and recirculate for 30 minutes.
Two postwashes were performed using 1.5 kg diafiltration buffer each time.
After 10 minutes, the retentate was harvested from the reservoir and replaced with 30 ml diafiltration buffer.
The reservoir was filled with 75ml of fresh diafiltration buffer and recirculated for 30 minutes.
An 80 % permeate replacement rate was established, using 4 % lactose in PBS as the diafiltration buffer.
The retentate was diafilteced with 3 volumes of Diafiltration Buffer ( below ) to produce the clarified lysate.
The column was washed with several bed-volumes of diafiltration buffer pH 8.0.
In a preferred embodiment, the diafiltration buffer contains at or about 0.25 M glycine.
See also
The material is then diafiltered against 5 volumes of Diafiltration buffer ( Formula 10 ).
The retentate was diafiltered with 3 volumes of Diafiltration Buffer ( below ) to produce the clarified lysate.
The charged membranes were equilibrated in the first diafiltration buffer for this experiment ( see Table 7 ).
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Examples of using Diafiltration
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Purification by dialysis or diafiltration can also be used
Diafiltration with buffer change of fermentation supernatant
Purification by dialysis or diafiltration may also be used
Examples of using Buffer
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No buffer zones are defined for the site
The boundary of the buffer zone is adequate
The buffer has had too much
